Human Muscarinic Acetylcholine Receptor ELISA Kit | M-AChR elisa kit

Human Muscarinic Acetylcholine Receptor ELISA Kit

Cat. Num. AAA765487

• Gene Names: CHRM1; M1; HM1; M1R
• Reactivity: Human
• Synonyms: Muscarinic Acetylcholine Receptor; Human Muscarinic Acetylcholine Receptor ELISA Kit; M-AChR; M-AChR elisa kit

• Reactivity: Human
• Sequence Length: 460

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for M-AChR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human M-AChR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human M-AChR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human M-AChR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human M-AChR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 297406
• NCBI GeneID: 1128
• UniProt Accession #: P11229; Q96RH1
• Molecular Weight: 50,748 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 1
• NCBI Official Symbol: CHRM1
• NCBI Official Synonym Symbols: M1; HM1; M1R
• NCBI Protein Information: muscarinic acetylcholine receptor M1
• UniProt Protein Name: Muscarinic acetylcholine receptor M1
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM1
• UniProt Entry Name: ACM1_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR m1: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: asymmetric synapse; cell junction; dendrite; integral to plasma membrane; membrane; nerve terminal; plasma membrane; postsynaptic density; postsynaptic membrane; synapse

Molecular Function: drug binding; G-protein coupled acetylcholine receptor activity; phosphoinositide phospholipase C activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; cell proliferation; cognition; entrainment of circadian clock; G-protein coupled receptor protein signaling pathway; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; nervous system development; neuromuscular synaptic transmission; phototransduction, visible light; positive regulation of cell proliferation; positive regulation of ion transport; protein kinase C activation; protein modification process; regulation of locomotion; regulation of vascular smooth muscle contraction; saliva secretion; signal transduction; synaptic transmission, cholinergic