Rabbit DEPDC5 Polyclonal Antibody | anti-DEPDC5 antibody

Phospho-DEPDC5 (Thr1531) Antibody

Cat. Num. AAA9614526

• Gene Names: DEPDC5; DEP.5; FFEVF
• Reactivity: Human; Predicted Reactivity: Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: DEPDC5; Polyclonal Antibody; Phospho-DEPDC5 (Thr1531) Antibody; DEP domain containing 5; DEP domain-containing protein 5; DEP.5; DEPD5_HUMAN; FFEVF; KIAA0645; anti-DEPDC5 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-DEPDC5 (Thr1531) Antibody detects endogenous levels of DEPDC5 only when phosphorylated at T1531.; Tissue Specificity: Expressed in developing and adult brain.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DEPDC5 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human DEPDC5 around the phosphorylation site of T1531.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Ubiquitinated. Amino acid-induced 'Lys-48'-linked polyubiquitination of DEPDC5 by the BCR (KLHL22) ubiquitin ligase complex leads to DEPDC5 proteasomal degradation and inhibition of the GATOR1 complex. Ubiquitination may occur at multiple lysines.
• Subunit Structure: Within the GATOR complex, component of the GATOR1 subcomplex, made of DEPDC5, NPRL2 and NPRL3. GATOR1 mediates the strong interaction of the GATOR complex with RRAGA/RRAGC and RRAGB/RRAGC heterodimers. Interacts (via DEP domain) with KLHL22; the interaction depends on amino acid availability.
• Similarity: The DEP domain mediates the interaction with KLHL22.Belongs to the IML1 family.
• Subcellular Location: Cytoplasm>Cytosol. Cytoplasm>Perinuclear region. Lysosome membrane.; Note: Localization to lysosomes is amino acid-independent.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using Phospho-DEPDC5(Thr1531) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Hela, using Phospho-DEPDC5(T1531) Antibody.)

Related Product Information for anti-DEPDC5 antibody

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 510120820
• NCBI GeneID: 9681
• UniProt Accession #: O75140; Q5K3V5; Q5THY9; Q5THZ0; Q5THZ1; Q5THZ3; Q68DR1; Q6MZX3; A6H8V6; A8MPX9; B4DH93; B9EGN9
• Molecular Weight: 177,910 Da
• NCBI Official Full Name: DEP domain-containing protein 5
• NCBI Official Synonym Full Names: DEP domain containing 5
• NCBI Official Symbol: DEPDC5
• NCBI Official Synonym Symbols: DEP.5; FFEVF
• NCBI Protein Information: DEP domain-containing protein 5
• UniProt Protein Name: DEP domain-containing protein 5
• Protein Family: DEP domain-containing protein
• UniProt Gene Name: DEPDC5
• UniProt Synonym Gene Names: KIAA0645
• UniProt Entry Name: DEPD5_HUMAN

NCBI Description

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytosol; lysosomal membrane; lysosome; perinuclear region of cytoplasm

Molecular Function: GTPase activator activity; protein complex binding

Biological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway; positive regulation of GTPase activity

Disease: Epilepsy, Familial Focal, With Variable Foci