Western Blot (WB)
(Western blot analysis of extracts from U251 MG, using C7orf59 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit C7orf59 Polyclonal Antibody | anti-LAMTOR4 antibody
C7orf59 Antibody
Gene Names
LAMTOR4; C7orf59
Reactivity
Human, RatPredicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
C7orf59; Polyclonal Antibody; C7orf59 Antibody; AV006840; CG059_HUMAN; Chromosome 7 open reading frame 59; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4; Ragulator complex protein LAMTOR4; UPF0539 protein C7orf59; anti-LAMTOR4 antibody
Host
Rabbit
Reactivity
Human, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
C7orf59 Antibody detects endogenous levels of total C7orf59.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
99
Applicable Applications for anti-LAMTOR4 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human C7orf59, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from U251 MG, using C7orf59 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from U251 MG, using C7orf59 Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612455 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612455 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-LAMTOR4 antibody
Function: As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated.
Subcellular Location: Lysosome.
Subunit Structure: Part of the Ragulator complex composed of LAMTOR1, LAMTOR2, LAMTOR3, LAMTOR4 and LAMTOR5. LAMTOR4 and LAMTOR5 form a heterodimer that interacts, through LAMTOR1, with a LAMTOR2, LAMTOR3 heterodimer. The Ragulator complex interacts with both the mTORC1 complex and heterodimers constituted of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; regulated by amino acid availability. The Ragulator complex interacts with SLC38A9; the probable amino acid sensor (PubMed:25561175, PubMed:25567906).
Similarity: Belongs to the LAMTOR4 family.
Subcellular Location: Lysosome.
Subunit Structure: Part of the Ragulator complex composed of LAMTOR1, LAMTOR2, LAMTOR3, LAMTOR4 and LAMTOR5. LAMTOR4 and LAMTOR5 form a heterodimer that interacts, through LAMTOR1, with a LAMTOR2, LAMTOR3 heterodimer. The Ragulator complex interacts with both the mTORC1 complex and heterodimers constituted of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; regulated by amino acid availability. The Ragulator complex interacts with SLC38A9; the probable amino acid sensor (PubMed:25561175, PubMed:25567906).
Similarity: Belongs to the LAMTOR4 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)10kD.
Predicted Molecular Weight: (Calculated)11kDa.
Predicted Molecular Weight: (Calculated)11kDa.
NCBI Official Full Name
ragulator complex protein LAMTOR4 isoform 1
NCBI Official Synonym Full Names
late endosomal/lysosomal adaptor, MAPK and MTOR activator 4
NCBI Official Symbol
LAMTOR4
NCBI Official Synonym Symbols
C7orf59
NCBI Protein Information
ragulator complex protein LAMTOR4
UniProt Protein Name
Ragulator complex protein LAMTOR4
Protein Family
UniProt Gene Name
LAMTOR4
UniProt Synonym Gene Names
C7orf59
UniProt Entry Name
LTOR4_HUMAN
Uniprot Description
LAMTOR4: Belongs to the UPF0539 family.
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: intracellular membrane-bound organelle; lysosomal membrane; lysosome
Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; protein complex scaffold
Biological Process: cell cycle arrest; macroautophagy; positive regulation of TOR signaling pathway; regulation of cell size
Research Articles on LAMTOR4
Similar Products
Product Notes
The LAMTOR4 lamtor4 (Catalog #AAA9612455) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C7orf59 Antibody reacts with Human, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's C7orf59 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the LAMTOR4 lamtor4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C7orf59, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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