Rabbit VPS33A Polyclonal Antibody | anti-VPS33A antibody

VPS33A Antibody

Cat. Num. AAA9611543

• Gene Names: VPS33A; MPSPS
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: VPS33A; Polyclonal Antibody; VPS33A Antibody; FLJ22395; FLJ23187; hVPS 33A; hVPS33A; Vacuolar protein sorting 33 homolog A (S. cerevisiae); Vacuolar protein sorting 33 homolog A; Vacuolar protein sorting 33A (rat homolog); Vacuolar protein sorting 33A (yeast); Vacuolar protein sorting 33A; Vacuolar protein sorting associated protein 33A; VPS 33A; anti-VPS33A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VPS33A Antibody detects endogenous levels of VPS33A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 596

• Applicable Applications for anti-VPS33A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human VPS33A.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endosome; Lysosome
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using VPS33A Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-VPS33A antibody

Description: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39 (PubMed:25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed:24554770).

Subunit Structure: Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:23351085, PubMed:25783203, PubMed:25266290, PubMed:23901104, PubMed:28013294). Interacts with RAB5C (By similarity). Interacts with UVRAG, STX17, MON1A and MON1B (PubMed:18552835, PubMed:20434987, PubMed:24554770, PubMed:28013294). Interacts with VIPAS39; however, this interaction is debated (PubMed:19109425, PubMed:23901104). Associates with adaptor protein complex 3 (AP-3) and clathrin (PubMed:21411634).

Similarity: Belongs to the STXBP/unc-18/SEC1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 18105056
• NCBI GeneID: 65082
• NCBI Accession #: NP_075067
• NCBI GenBank Nucleotide #: NP_075067.2
• UniProt Accession #: Q96AX1
• Molecular Weight: Observed: 68 kDa; Predicted: 68 kDa
• NCBI Official Full Name: vacuolar protein sorting-associated protein 33A isoform 1
• NCBI Official Synonym Full Names: VPS33A core subunit of CORVET and HOPS complexes
• NCBI Official Symbol: VPS33A
• NCBI Official Synonym Symbols: MPSPS
• NCBI Protein Information: vacuolar protein sorting-associated protein 33A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 33A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS33A
• UniProt Synonym Gene Names: hVPS33A
• UniProt Entry Name: VP33A_HUMAN

NCBI Description

This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]