Rabbit MARS Polyclonal Antibody | anti-MARS antibody

MARS Antibody

Cat. Num. AAA9611888

• Gene Names: MARS1; MRS; ILLD; MARS; CMT2U; ILFS2; METRS; MTRNS; SPG70
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MARS; Polyclonal Antibody; MARS Antibody; cytoplasmic; FLJ35667; Mars; Methionine tRNA ligase 1; Methionine tRNA ligase 1 cytoplasmic; Methionine tRNA ligase; Methionine tRNA synthetase; Methionine--tRNA ligase; Methionyl tRNA synthetase cytoplasmic; Methionyl-tRNA synthetase; MetRS; MRS; MTRNS; SYMC_HUMAN; anti-MARS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MARS Antibody detects endogenous levels of MARS.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 900

• Applicable Applications for anti-MARS antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human MARS.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Cytosol
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using MARS Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MARS antibody

Description: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Subunit Structure: Monomer (PubMed:11714285). Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:19131329, PubMed:19289464, Ref. 22, PubMed:26472928). Forms a linear complex that contains MARS, EEF1E1, EPRS and AIMP2 that is at the core of the multisubunit complex (PubMed:26472928).

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family.

NCBI and Uniprot Product Information

• NCBI GI #: 14043022
• NCBI GeneID: 4141
• NCBI Accession #: NP_004981
• NCBI GenBank Nucleotide #: NP_004981.2
• UniProt Accession #: P56192
• Molecular Weight: Observed: 101 kDa; Predicted: 102 kDa
• NCBI Official Full Name: methionine--tRNA ligase, cytoplasmic
• NCBI Official Synonym Full Names: methionyl-tRNA synthetase 1
• NCBI Official Symbol: MARS1
• NCBI Official Synonym Symbols: MRS; ILLD; MARS; CMT2U; ILFS2; METRS; MTRNS; SPG70
• NCBI Protein Information: methionine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Methionine--tRNA ligase, cytoplasmic
• Protein Family: Methionine--tRNA ligase
• UniProt Gene Name: MARS
• UniProt Synonym Gene Names: MetRS
• UniProt Entry Name: SYMC_HUMAN

NCBI Description

This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]