Rabbit anti-Human DEPDC5 Polyclonal Antibody | anti-DEPDC5 antibody

DEPDC5 Antibody

Cat. Num. AAA9610390

• Gene Names: DEPDC5; DEP.5; FFEVF; FFEVF1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DEPDC5; Polyclonal Antibody; DEPDC5 Antibody; DEP domain containing 5; DEP domain-containing protein 5; DEP.5; DEPD5_HUMAN; FFEVF; KIAA0645; anti-DEPDC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DEPDC5 Antibody detects endogenous levels of DEPDC5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1603

• Applicable Applications for anti-DEPDC5 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DEPDC5.
• Tissue Specificity: Expressed in developing and adult brain.
• Subcellular Location: Lysosome; Cytosol
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of DEPDC5 expression in HeLa lysate)

Related Product Information for anti-DEPDC5 antibody

Description: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.

Subunit Structure: Within the GATOR complex, component of the GATOR1 subcomplex, made of DEPDC5, NPRL2 and NPRL3. GATOR1 mediates the strong interaction of the GATOR complex with RRAGA/RRAGC and RRAGB/RRAGC heterodimers.

Similarity: Belongs to the IML1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 510120820
• NCBI GeneID: 9681
• UniProt Accession #: O75140
• Molecular Weight: Observed: 180 kDa; Predicted: 182 kDa
• NCBI Official Full Name: GATOR complex protein DEPDC5
• NCBI Official Synonym Full Names: DEP domain containing 5
• NCBI Official Symbol: DEPDC5
• NCBI Official Synonym Symbols: DEP.5; FFEVF; FFEVF1
• NCBI Protein Information: GATOR complex protein DEPDC5
• UniProt Protein Name: DEP domain-containing protein 5
• Protein Family: DEP domain-containing protein
• UniProt Gene Name: DEPDC5
• UniProt Synonym Gene Names: KIAA0645
• UniProt Entry Name: DEPD5_HUMAN

NCBI Description

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytosol; lysosomal membrane; lysosome; perinuclear region of cytoplasm

Molecular Function: GTPase activator activity; protein complex binding

Biological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway; positive regulation of GTPase activity

Disease: Epilepsy, Familial Focal, With Variable Foci