Western Blot (WB)
(Western blot analysis of TUSC4 using HepG2 whole cell lysates)
Rabbit TUSC4 Polyclonal Antibody | anti-TUSC4 antibody
TUSC4 Antibody
Gene Names
NPRL2; NPR2; NPR2L; TUSC4; FFEVF2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TUSC4; Polyclonal Antibody; TUSC4 Antibody; 2810446G01Rik; G21 protein; Gene 21 protein; Homologous to yeast nitrogen permease (candidate tumor suppressor); Homologous to yeast nitrogen permease; Nitrogen permease regulator 2-like protein; Nitrogen permease regulator-like 2 (S. cerevisiae); NPR 2L; NPR L2; NPR like 2; NPR2; NPR2 like; NPR2-like protein; NPR2L; NPRL 2; NPRL2; NPRL2_HUMAN; Tumor suppressor candidate 4; TUSC 4; TUSC 4 protein; TUSC4 protein; anti-TUSC4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TUSC4 antibody detects endogenous levels of total TUSC4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
380
Applicable Applications for anti-TUSC4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human TUSC4
Subcellular Location
Lysosome
Tissue Specificity
Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes (PubMed:27173016, PubMed:26505888). Expressed in most lung cancer cell lines tested.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (90%), Zebrafish (100%), Bovine (90%), Horse (100%), Sheep (90%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of TUSC4 using HepG2 whole cell lysates)
Western Blot (WB)
(Western blot analysis of TUSC4 using HepG2 whole cell lysates)
Related Product Information for anti-TUSC4 antibody
Function: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
Subunit Structure: Forms a heterodimer with NPRL3. Interacts with PDPK1. Within the GATOR complex, component of the GATOR1 subcomplex, made of DEPDC5, NPRL2 and NPRL3. GATOR1 mediates the strong interaction of the GATOR complex with RRAGA/RRAGC and RRAGB/RRAGC heterodimers.
Similarity: Belongs to the NPR2 family.
Subunit Structure: Forms a heterodimer with NPRL3. Interacts with PDPK1. Within the GATOR complex, component of the GATOR1 subcomplex, made of DEPDC5, NPRL2 and NPRL3. GATOR1 mediates the strong interaction of the GATOR complex with RRAGA/RRAGC and RRAGB/RRAGC heterodimers.
Similarity: Belongs to the NPR2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 44 kDa
Predicted: 44 kDa
Predicted: 44 kDa
NCBI Official Full Name
GATOR complex protein NPRL2
NCBI Official Synonym Full Names
NPR2 like, GATOR1 complex subunit
NCBI Official Symbol
NPRL2
NCBI Official Synonym Symbols
NPR2; NPR2L; TUSC4; FFEVF2
NCBI Protein Information
GATOR complex protein NPRL2
UniProt Protein Name
GATOR complex protein NPRL2
UniProt Gene Name
NPRL2
UniProt Synonym Gene Names
G21 protein1 PublicationManual assertion based on opinion iniRef.1; NPR2-like protein1 PublicationManual assertion based on opinion iniRef.7
Uniprot Description
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
Research Articles on TUSC4
Similar Products
Product Notes
The TUSC4 nprl2 (Catalog #AAA9602928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TUSC4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TUSC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the TUSC4 nprl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUSC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
