Goat HCST Polyclonal Antibody | anti-Hcst antibody

HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10, Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP, UNQ587/PRO1157)

Cat. Num. AAA6006153

• Gene Names: Hcst; DAP10; KAP10
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: HCST; Polyclonal Antibody; HCST (DAP10; Hematopoietic Cell Signal Transducer; DNAX-activation Protein 10; Membrane Protein DAP10; Transmembrane Adapter Protein KAP10; KAP10; PIK3AP; UNQ587/PRO1157); Anti -HCST (DAP10; anti-Hcst antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human HCST. Species sequence homology: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-Hcst antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:128,000; Western Blot: 0.2-0.6ug/ml
• Immunogen: Synthetic peptide corresponding to C-RSPAQEDGKVYIN, from human HCST, at C-terminal (NP_055081.1, NP_001007470.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of human spleen lysate (35ug protein in RIPA buffer) using MBS6006153 (0.2ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-Hcst antibody

Transmembrane adapter protein which associates with NKG2D to form an activation receptor NKG2D-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with NKG2D-DAP10 triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full NKG2D-HCST-mediated activation and ultimate killing of target cells. In NK cells, NKG2D-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. NKG2D-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express NKG2D ligands escape from immune surveillance mediated by NK cells.

Product Categories/Family for anti-Hcst antibody

Antibodies; Abs to Adapter Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 219519586
• NCBI GeneID: 23900
• UniProt Accession #: Q9QUJ0; Q9R1E7
• Molecular Weight: 8,114 Da
• NCBI Official Full Name: Hcst protein
• NCBI Official Synonym Full Names: hematopoietic cell signal transducer
• NCBI Official Symbol: Hcst
• NCBI Official Synonym Symbols: DAP10; KAP10
• NCBI Protein Information: hematopoietic cell signal transducer; membrane protein DAP10; DNAX-activation protein 10; transmembrane adapter protein KAP10
• UniProt Protein Name: Hematopoietic cell signal transducer
• Protein Family: Hematopoietic cell signal transducer
• UniProt Gene Name: Hcst
• UniProt Synonym Gene Names: Dap10; Kap10
• UniProt Entry Name: HCST_MOUSE

Uniprot Description

HCST: Transmembrane adapter protein which associates with NKG2D to form an activation receptor NKG2D-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with NKG2D-DAP10 triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full NKG2D-HCST-mediated activation and ultimate killing of target cells. In NK cells, NKG2D-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. NKG2D-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express NKG2D ligands escape from immune surveillance mediated by NK cells. Belongs to the DAP10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane

Molecular Function: protein binding; phosphoinositide 3-kinase binding; receptor binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade