Goat anti-Human HCST Polyclonal Antibody | anti-HCST antibody

HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10, Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP, UNQ587/PRO1157) (Biotin)

Cat. Num. AAA6479540

• Gene Names: HCST; DAP10; KAP10; PIK3AP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: HCST; Polyclonal Antibody; HCST (DAP10; Hematopoietic Cell Signal Transducer; DNAX-activation Protein 10; Membrane Protein DAP10; Transmembrane Adapter Protein KAP10; KAP10; PIK3AP; UNQ587/PRO1157) (Biotin); anti-HCST antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HCST. Recognizes isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking the residue E81 (NP_001007470.1).
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HCST antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to C-RSPAQEDGKVYIN, from the C-termainal of human HCST. Species sequence homology: mouse, rat
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of human spleen lysate (35ug protein in RIPA buffer) using MBS6006153 (0.2ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-HCST antibody

Transmembrane adapter protein which associates with NKG2D to form an activation receptor NKG2D-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with NKG2D-DAP10 triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full NKG2D-HCST-mediated activation and ultimate killing of target cells. In NK cells, NKG2D-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. NKG2D-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express NKG2D ligands escape from immune surveillance mediated by NK cells.

Product Categories/Family for anti-HCST antibody

Antibodies; Adapter Proteins

References

1. Upshaw JL, Arneson LN, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ. NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells. Nat Immunol. 2006 May;7(5):524-32. Epub 2006 Apr 2.

NCBI and Uniprot Product Information

• NCBI GI #: 15826850
• NCBI GeneID: 10870
• NCBI Accession #: NP_055081.1; NP_001007470.1
• NCBI GenBank Nucleotide #: NM_014266.3
• NCBI Official Full Name: hematopoietic cell signal transducer isoform 1
• NCBI Official Synonym Full Names: hematopoietic cell signal transducer
• NCBI Official Symbol: HCST
• NCBI Official Synonym Symbols: DAP10; KAP10; PIK3AP
• NCBI Protein Information: hematopoietic cell signal transducer
• Protein Family: Hematopoietic cell signal transducer

NCBI Description

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]