DAP10 Recombinant Protein | HCST recombinant protein

DAP10 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121618

• Gene Names: HCST; DAP10; KAP10; PIK3AP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DAP10; DAP10 Protein; Human; Recombinant (mFc Tag); Human HCST/DAP10 Protein (Fc Tag); KAP10 Protein; PIK3AP Protein; hematopoietic cell signal transducer; HCST recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro48

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 19
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human HCST (Q9UBK5-1)(Met-Pro48) was fused with Fc region of mouse IgG at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HCST recombinant protein

Background: HCST (Hematopoietic Cell Signal Transducer, also known as DAP1) is a Protein Coding gene. HCST belongs to the DAP10 family. It is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. HCST may be part of the immune recognition receptor complex. This receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs). It may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif.

References

Wu J. et al., 1999, Science. 285 (5428): 730-2.Karimi M. et al., 2006, J Immunol. 175 (12): 7819-28.Andra© P. et al., 2004, Eur J Immunol. 34 (4): 961-71.

NCBI and Uniprot Product Information

• NCBI GI #: 74734930
• NCBI GeneID: 10870
• UniProt Accession #: Q9UBK5; Q9UBS1; Q9Y3Y0
• Molecular Weight: 9,360 Da
• NCBI Official Full Name: Hematopoietic cell signal transducer
• NCBI Official Synonym Full Names: hematopoietic cell signal transducer
• NCBI Official Symbol: HCST
• NCBI Official Synonym Symbols: DAP10; KAP10; PIK3AP
• NCBI Protein Information: hematopoietic cell signal transducer
• UniProt Protein Name: Hematopoietic cell signal transducer
• Protein Family: Hematopoietic cell signal transducer
• UniProt Gene Name: HCST
• UniProt Synonym Gene Names: DAP10; KAP10; PIK3AP

NCBI Description

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells.