HCST recombinant protein

HCST, 20-48aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206794

• Gene Names: HCST; DAP10; KAP10; PIK3AP
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: HCST; 20-48aa; Human; hIgG-His-tag; Baculovirus; Hematopoietic cell signal transducer isoform 1; DAP10; KAP10; PIK3AP; HCST recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPTTPGERS SLPAFYPGTS GSCSGCGSLS LPLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H
• Sequence Length: 93

• Applicable Applications for HCST recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HCST recombinant protein

HCST, also known as hematopoietic cell signal transducer isoform 1, is a member of the DAP10 family. It can be part of an immunoreceptor complex. This receptor plays an important role in inducing cytotoxicity against MHC class I chain-associated MICA and target cells expressing cell surface ligands such as MICB and UL16-binding protein (ULBP). This receptor complex can play a role in cell survival and proliferation by activation of NK and T cell responses.Recombinant human HCST, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for HCST recombinant protein

Signal Transduction

References

Karimi M., et al. (2006) J Immunol. 175:7819-7828.; Andre P., et al. (2004) Eur J Immunol. 34:961-971.

NCBI and Uniprot Product Information

• NCBI GI #: 15826850
• NCBI GeneID: 10870
• NCBI Accession #: NP_055081
• NCBI GenBank Nucleotide #: NM_014266.3
• UniProt Accession #: Q9UBK5; Q9UBS1; Q9Y3Y0
• Molecular Weight: 30.1kDa (271aa) 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: hematopoietic cell signal transducer isoform 1
• NCBI Official Synonym Full Names: hematopoietic cell signal transducer
• NCBI Official Symbol: HCST
• NCBI Official Synonym Symbols: DAP10; KAP10; PIK3AP
• NCBI Protein Information: hematopoietic cell signal transducer
• UniProt Protein Name: Hematopoietic cell signal transducer
• Protein Family: Hematopoietic cell signal transducer
• UniProt Gene Name: HCST
• UniProt Synonym Gene Names: DAP10; KAP10; PIK3AP

NCBI Description

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells.