Chicken Lymphocyte function associated antigen 3 ELISA Kit | LFA-3 elisa kit

Chicken Lymphocyte function associated antigen 3 ELISA Kit

Cat. Num. AAA740977

• Gene Names: CD58; ag3; LFA3; LFA-3
• Reactivity: Chicken
• Synonyms: Lymphocyte function associated antigen 3; Chicken Lymphocyte function associated antigen 3 ELISA Kit; LFA-3 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This LFA-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken LFA-3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LFA-3 elisa kit

Principle of the Assay: LFA-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LFA-3 antibody and an LFA-3-HRP conjugate. The assay sample and buffer are incubated together with LFA-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LFA-3 concentration since LFA-3 from samples and LFA-3-HRP conjugate compete for the anti-LFA-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by LFA-3 from the sample, fewer sites are left to bind LFA-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LFA-3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LFA-3 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4502677
• NCBI GeneID: 965
• NCBI Accession #: NP_001770.1
• NCBI GenBank Nucleotide #: NM_001779.2
• UniProt Accession #: P19256; Q5U053; Q6IB65; Q96KI9; A8K7G5; Q14748; Q16393; Q9BRW0
• Molecular Weight: 28,147 Da
• NCBI Official Full Name: lymphocyte function-associated antigen 3 isoform 1
• NCBI Official Synonym Full Names: CD58 molecule
• NCBI Official Symbol: CD58
• NCBI Official Synonym Symbols: ag3; LFA3; LFA-3
• NCBI Protein Information: lymphocyte function-associated antigen 3; surface glycoprotein LFA-3; CD58 antigen, (lymphocyte function-associated antigen 3)
• UniProt Protein Name: Lymphocyte function-associated antigen 3
• UniProt Gene Name: CD58
• UniProt Synonym Gene Names: LFA3; Ag3
• UniProt Entry Name: LFA3_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell adhesion; blood coagulation; leukocyte migration