Mouse anti-Human KLRK1 Monoclonal Antibody | anti-Klrk1 antibody

KLRK1 (Killer Cell Lectin-like Receptor Subfamily K Member 1, CD314, HGNC:18788, D12S2489E, NK Cell Receptor D, NK Lectin-like Receptor, NKLLR, NKG2D Activating NK Receptor, NKG2D Type II Integral Membrane Protein, NKR P2)

Cat. Num. AAA606386

• Gene Names: Klrk1; Nkg2d; NKG2-D; D6H12S2489E
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: KLRK1; Monoclonal Antibody; KLRK1 (Killer Cell Lectin-like Receptor Subfamily K Member 1; CD314; HGNC:18788; D12S2489E; NK Cell Receptor D; NK Lectin-like Receptor; NKLLR; NKG2D Activating NK Receptor; NKG2D Type II Integral Membrane Protein; NKR P2); Anti -KLRK1 (Killer Cell Lectin-like Receptor Subfamily K Member 1; anti-Klrk1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B1388
• Specificity: Recognizes human CD314. Reported to inhibit T cell recognition of MICA (1,6).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4. No preservatives added.

• Applicable Applications for anti-Klrk1 antibody: Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Functional Assays, Immunohistochemistry (frozen), and Immunoprecipitation.; Dilution: Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul
• Immunogen: NKL cells.
• Hybridoma: P3 myeloma cells with spleen cells from RBF/DnJ mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Staining of human peripheral blood lymphocytes with MBS606386.)

Testing Data

Testing Data

Related Product Information for anti-Klrk1 antibody

CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein.Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells.

Product Categories/Family for anti-Klrk1 antibody

Antibodies; Abs to Cell Markers

NCBI and Uniprot Product Information

• NCBI GI #: 71060095
• NCBI GeneID: 27007
• UniProt Accession #: Q4FJM0; O54709; Q2TJJ6; Q3TCW7
• Molecular Weight: 26,710 Da
• NCBI Official Full Name: Klrk1
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily K, member 1
• NCBI Official Symbol: Klrk1
• NCBI Official Synonym Symbols: Nkg2d; NKG2-D; D6H12S2489E
• NCBI Protein Information: NKG2-D type II integral membrane protein; NK cell receptor D; natural killer cell group 2D; NKG2-D-activating NK receptor
• UniProt Protein Name: Klrk1 protein
• Protein Family: NKG2-D type II integral membrane protein
• UniProt Gene Name: Klrk1
• UniProt Synonym Gene Names: Nkg2d
• UniProt Entry Name: Q4FJM0_MOUSE

Uniprot Description

KLRK1: Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: MHC class Ib receptor activity; protein binding; protein homodimerization activity; MHC class I protein binding; carbohydrate binding; kinase binding

Biological Process: stimulatory C-type lectin receptor signaling pathway; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; positive regulation of nitric oxide biosynthetic process; immune system process; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of apoptosis; innate immune response; natural killer cell activation; positive regulation of natural killer cell mediated cytotoxicity; cell differentiation; positive regulation of myeloid dendritic cell activation