Rabbit GABAA Receptor a 5 Polyclonal Antibody | anti-GABRA5 antibody

Anti-GABAA Receptor, alpha5-Subunit

Cat. Num. AAA502047

• Reactivity: Tested: Mouse, rat. Predicted: Bovine, Canine, Chicken, Human, Non-human Primate, Zebrafish
• Applications: Dot Blot, Immunohistochemistry, Western Blot
• Purity: Affinity purified rabbit polyclonal antibody. Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Synonyms: GABAA Receptor a 5; Polyclonal Antibody; Anti-GABAA Receptor; alpha5-Subunit; anti-GABRA5 antibody

• Host: Rabbit
• Reactivity: Tested: Mouse, rat. Predicted: Bovine, Canine, Chicken, Human, Non-human Primate, Zebrafish
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~55 kDa alpha5-subunit of the GABAA receptor.
• Purity/Purification: Affinity purified rabbit polyclonal antibody. Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol.
• Sequence Length: 464

• Applicable Applications for anti-GABRA5 antibody: Western Blot (WB); Dot Blots (DB). Suitability for Immunohistochemistry (IHC) not yet determined.
• Application Notes: Quality Control: Dot Blots & Western blots performed on each lot.; ; Working Dilutions: WB 1:500
• Antigen: Fusion protein from the cytoplasmic loop of the alpha5-subunit of rat GABAA receptor.
• Preparation and Storage: Shipped on blue ice. Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20 degree C.

Western Blot (WB)

(Western blot of mouse whole brain showing specific immunolabeling of the ~55 kDa alpha5-subunit of the GABAA-R.)

Related Product Information for anti-GABRA5 antibody

Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl? channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six ?s, four ?s and four ?s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for ?- and ?-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a ?-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different ?-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

References

Yu, J., Wang, D.S., Bonin, R.P., Penna, A., Alavian-Ghavanini, A., Zurek, A.A., Rauw, G., Baker, G.B. and Orser, B.A., 2019. Gabapentin increases expression of ? subunit-containing GABAA receptors. EBioMedicine, pii: S2352-3964(19)30148-3.
Engin, E., Zarnowska, E. D., Benke, D., Tsvetkov, E., Sigal, M., Keist, R., Bolshakov, V.Y., Pearce, R.A., & Rudolph, U. (2015). Tonic Inhibitory Control of Dentate Gyrus Granule Cells by ?5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.
Centanni, S. W., Teppen, T., Risher, M. L., Fleming, R. L., Moss, J. L., Acheson, S. K., Mulholland, P.J., Pandey, S.C., Chandler, L.J., & Swartzwelder, H. S. (2014). Adolescent alcohol exposure alters GABAA receptor subunit expression in adult hippocampus. Alcoholism: Clinical and Experimental Research, 38(11), 2800-2808.

NCBI and Uniprot Product Information

• NCBI GI #: 25742787
• NCBI GeneID: 29707
• NCBI Accession #: NP_058991.1
• NCBI GenBank Nucleotide #: NM_017295.1
• UniProt Accession #: P19969
• Molecular Weight: ~55 kDa
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit alpha-5
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, alpha 5
• NCBI Official Symbol: Gabra5
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit alpha-5; GABA-A receptor alpha-5 subunit; GABA(A) receptor subunit alpha-5; gamma-aminobutyric acid A receptor, alpha 5; gamma-aminobutyric acid A receptor, alpha 5; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 5
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit alpha-5
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: Gabra5
• UniProt Synonym Gene Names: Gabra-5
• UniProt Entry Name: GBRA5_RAT

NCBI Description

subunit of the GABAA receptor; may be part of distinct GABAA receptors within the olfactory bulb [RGD, Feb 2006]

Uniprot Description

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA5 sub-subfamily. [View classification]