Rabbit anti-Mouse, Rat GABAA Polyclonal Antibody | anti-GABAA antibody

Anti-GABAA Receptor, beta3-Subunit Antibody

Cat. Num. AAA500072

• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Synonyms: GABAA; Polyclonal Antibody; Anti-GABAA Receptor; beta3-Subunit Antibody; anti-GABAA antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~53 kDa beta3-subunit of the GABAA receptor.
• Purity/Purification: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Form/Format: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 473

• Applicable Applications for anti-GABAA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000
• Immunogen Species: Rat; Immunogen Information
• Antigen: Fusion protein from the cytoplasmic loop of the beta3-subunit of rat GABAA receptor.
• Preparation and Storage: Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.; Stable for at least 1 year at -20 degree C.

Western Blot (WB)

(Western blot of 5-7 mug of mouse cerebellar lysates from wild type (WT) and beta3 knockout (K/O) animals showing specific immunolabeling of the ~53 kDa beta3-subunit of the GABAA-R in the wild type but not in the beta3 K/O animals.)

Related Product Information for anti-GABAA antibody

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

References

Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455.
McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592.
Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653.
Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.

NCBI and Uniprot Product Information

• NCBI GI #: 8393390
• NCBI GeneID: 24922
• NCBI Accession #: NP_058761.1
• NCBI GenBank Nucleotide #: NM_017065.1
• UniProt Accession #: P63079; P15433
• Molecular Weight: ~53 kDa
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit beta-3
• NCBI Official Synonym Full Names: gamma-aminobutyric acid type A receptor beta 3 subunit
• NCBI Official Symbol: Gabrb3
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit beta-3
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit beta-3
• UniProt Gene Name: Gabrb3
• UniProt Synonym Gene Names: Gabrb-3

NCBI Description

beta 3 subunit, with alpha subunits forms active GABA-A receptors [RGD, Feb 2006]

Uniprot Description

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.