Chicken Arginase I/Liver Arginase (ARG1) Polyclonal Antibody | anti-ARG1 antibody

Anti-Arginase I/Liver Arginase (ARG1) Antibody

Cat. Num. AAA500082

• Reactivity: Guinea Pig, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Total IgY Fraction
• Synonyms: Arginase I/Liver Arginase (ARG1); Polyclonal Antibody; Anti-Arginase I/Liver Arginase (ARG1) Antibody; Arginase I (ARG1); anti-ARG1 antibody

• Host: Chicken
• Reactivity: Guinea Pig, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~35-38 kDa arginase I protein.
• Purity/Purification: Total IgY Fraction
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 294

• Applicable Applications for anti-ARG1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000; IHC: 1:500
• Immunogen Species: Rat
• Immunogen Information: Full-length recombinant rat arginase I.
• Antigen: Full-length recombinant rat liver arginase I expressed in and purified from E Coli.
• Quality Control Tests: Western blots performed on each lot.
• Preparation and Storage: Store at -20 degree C in undiluted aliquots as aliquots may be taken without freeze/thawing due to presence of 50% glycerol stable for at least 1 year.

Western Blot (WB)

(Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa arginase I protein.)

Related Product Information for anti-ARG1 antibody

Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996). Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007).

References

1. Morris SM, Kepka-Lenhart D, Chen LC. Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells. Am J Physiol 275(5 Pt 1):E740-7 (1998). 2. Poljakovic M, Porter DW, Millecchia L, Kepka-Lenhart D, Beighley C, Wolfarth MG, Castranova V, Morris SM. Celland Isoform-specific increases in arginase expression in acute silica-induced pulmonary inflammation. J Toxicol Environ Health A 15;70(2):118-127 (2007). 3. Jenkinson CP, Grody WW, Cederbaum SD. Comparative properties of arginases. Comp. Biochem. Physiol B Biochem Mol Biol 114B:107-132 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 319754464
• UniProt Accession #: P07824
• Molecular Weight: ~35,38 kDa
• NCBI Official Full Name: arginase
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: Arg1
• UniProt Entry Name: ARGI1_RAT

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.3.1; Amino Acid Metabolism - arginine and proline

Cellular Component: extracellular space; mitochondrial outer membrane; neuron projection; cell soma; cytoplasm; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: mammary gland involution; response to peptide hormone stimulus; maternal process involved in pregnancy; response to lipopolysaccharide; female pregnancy; response to vitamin A; response to selenium ion; response to vitamin E; response to methylmercury; response to wounding; response to axon injury; collagen biosynthetic process; aging; response to drug; response to herbicide; liver development; response to amino acid stimulus; response to manganese ion; response to cadmium ion; arginine metabolic process; response to zinc ion; response to steroid hormone stimulus; arginine catabolic process to ornithine; positive regulation of endothelial cell proliferation; lung development; response to amine stimulus; urea cycle