Rabbit FMRP (Ser499) Polyclonal Antibody | anti-FMR1 antibody

Anti-Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein)

Cat. Num. AAA502189

• Gene Names: Fmr1; FMRP
• Applications: Dot Blot, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: FMRP (Ser499); Polyclonal Antibody; Anti-Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein); anti-FMR1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Specific for ~71k FMRP protein phosphorylated at Ser⁴⁹⁹. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase.
• Purity/Purification: Affinity Purified
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots

• Applicable Applications for anti-FMR1 antibody: Western Blot (WB) and Dot blots (DB). Immunohistochemistry (IHC) has not yet been determined.
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; DB: 1:1000
• Preparation and Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.

Testing Data

Related Product Information for anti-FMR1 antibody

Affinity purified rabbit polyclonal antibody
Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

References

• Bernard PB, Castano AM, O'Leary H, Simpson K, Browning MD, Benke TA. (2013) Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures. Neurobiol Dis. Nov; 59:1-17.
• Brown V, Small K, Lakkis L, Feng Y, Gunter C, Wilkinson KD, Warren ST (1998) Purified recombinant Fmrp exhibits selective RNA binding as an intrinsic property of the fragile X mental retardation protein. J Biol Chem 273:15521-15527
• Ceman S, O'Donnell WT, Reed M, Patton S, Pohl J, Warren ST. (2003) Phosphorylation influences the translation state of FMRP-associated polyribosomes. Hum Mol Genet. Dec 15;12(24):3295-305
• Goodlin-Jones BL, Tassone F, Gane LW, Hagerman RJ. (2004) Autistic spectrum disorder and the fragile X premutation. J Dev Behav Pediatr. Dec;25(6):392-8
• Narayanan U, Nalavadi V, Nakamoto M, Thomas G, Ceman S, Bassell GJ, Warren ST. (2008) S6K1 phosphorylates and regulates fragile X mental retardation protein (FMRP) with the neuronal protein synthesis-dependent mammalian target of rapamycin (mTOR) signaling cascade. J Biol Chem 283:18478-18482

NCBI and Uniprot Product Information

• NCBI GI #: 30794228
• NCBI GeneID: 24948
• NCBI Accession #: NP_434691.1
• NCBI GenBank Nucleotide #: NM_052804.1
• UniProt Accession #: Q80WE1; P70568
• NCBI Official Full Name: fragile X mental retardation protein 1 homolog
• NCBI Official Synonym Full Names: fragile X mental retardation 1
• NCBI Official Symbol: Fmr1
• NCBI Official Synonym Symbols: FMRP
• NCBI Protein Information: fragile X mental retardation protein 1 homolog; protein FMR-1; fragile X mental retardation-1 protein; fragile X mental retardation syndrome 1 homolog
• UniProt Protein Name: Fragile X mental retardation protein 1 homolog
• Protein Family: Mat- sexual cell fertilization-promoting factor
• UniProt Gene Name: Fmr1
• UniProt Synonym Gene Names: FMRP
• UniProt Entry Name: FMR1_RAT

NCBI Description

may play a role in mRNA trafficking and localization [RGD, Feb 2006]

Uniprot Description

Function: Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression. RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. Involved in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C)

By similarity.

Subunit structure: Homooligomer. Found in a RNP granule complex with IGF2BP1. Directly interacts with SMN and TDRD3. Interacts with the SMN core complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 and RANBP9. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with CYFIP1 and CYFIP2. The interaction with brain cytoplasmic RNA 1 (BC1) increases binding affinity for the CYFIP1-EIF4E complex in the brain

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus › nucleolus

By similarity.

Domain: The tandem Tudor domains preferentially recognize trimethylated histone peptides

By similarity.

Post-translational modification: Phosphorylated on several serine residues

By similarity.

Miscellaneous: RNA-binding activity is inhibited by RANBP9

By similarity.

Sequence similarities: Belongs to the FMR1 family.Contains 2 Agenet-like domains.Contains 2 KH domains.

Sequence caution: The sequence AAB07073.1 differs from that shown. Reason: N-ter sequencing errors.