Rabbit Draxin Polyclonal Antibody | anti-DRAXIN antibody

Draxin (C-terminal region) Antibody

Cat. Num. AAA474303

• Gene Names: DRAXIN; UNQ3119; neucrin; AGPA3119; C1orf187
• Reactivity: Human, Rat, Mouse, Chicken
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: Draxin; Polyclonal Antibody; Draxin (C-terminal region) Antibody; Draxin (C-terminal region); anti-DRAXIN antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using Draxin (C-terminal region) peptide (without carrier). The antibody detects a significant 50kDa* protein corresponding to the molecular mass of draxin on SDS-PAGE immunoblots of rat PC12 cells, rat P1 brain, and chick E9 brain. The antibody also shows low expression of draxin in adult mouse brain.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-DRAXIN antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; WB: 1:1000
• Immunogen: Draxin synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human draxin. This peptide sequence is highly conserved in rat, mouse, and chick draxin.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of draxin expression in rat PC12 cells (lane 1), rat P1 brain (lane 2), adult mouse brain (lane 3), and chick E9 brain (lane 4). The blot was probed with rabbit polyclonal anti-Draxin (C-terminal region) at 1:1000.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of Draxin in rat PC12 cells differentiated with NGF. The cells were probed with Draxin (C-terminal region) rabbit polyclonal antibody, then the antibody was detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (DX3675). Lower images show corresponding phase images.)

Related Product Information for anti-DRAXIN antibody

The developing nervous system assembles with the help of guidance molecules that control the trajectory of growing neurons. Axon guidance proteins such as, netrins, semaphorins, ephrins, and slits, are critical extracellular matrix cues for correctly wiring of the nervous system. Draxin is an axon guidance protein that has been observed to repel neurite outgrowth from dorsal spinal cord and cortical explants in vitro. Chick draxin consists of 349 amino acids with a putative signal peptide sequence at the N-terminal end but no membrane anchoring sequence, which suggests that draxin is a secreted protein. The ectopically expressed draxin has been shown to inhibit growth or cause misrouting of chick spinal cord commissural axons in vivo. In both chick and mouse, commissural neurons require draxin to successfully cross the midline and form the major commissures of the brain. Thus, draxin is a chemorepulsive axon guidance molecule required for the development of the spinal cord and forebrain commissures.

References

Hines, P. J. (2009) Sci. Signal. 2(54):ec23.
Islam, S. M. et al. (2009) Science 323(5912):388.

NCBI and Uniprot Product Information

• NCBI GI #: 86990448
• NCBI GeneID: 374946
• NCBI Accession #: BAG80561
• NCBI GenBank Nucleotide #: NM_198545.3
• UniProt Accession #: Q8NBI3; Q5SNX0; Q6UWK8; B6EV15
• Molecular Weight: 38,650 Da
• NCBI Official Full Name: draxin
• NCBI Official Synonym Full Names: dorsal inhibitory axon guidance protein
• NCBI Official Symbol: DRAXIN
• NCBI Official Synonym Symbols: UNQ3119; neucrin; AGPA3119; C1orf187
• NCBI Protein Information: draxin; dorsal repulsive axon guidance protein; dorsal inhibitory axon guidance protein; neural tissue-specific cysteine-rich protein
• UniProt Protein Name: Draxin
• Protein Family: Draxin
• UniProt Gene Name: DRAXIN
• UniProt Entry Name: DRAXI_HUMAN

Uniprot Description

DRAXIN: Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta- catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway. Belongs to the draxin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: extracellular region

Biological Process: dorsal spinal cord development; axon guidance; Wnt receptor signaling pathway; forebrain development; commissural neuron differentiation in the spinal cord; negative regulation of neuron apoptosis; negative regulation of axon extension