Rabbit NEUCRIN Polyclonal Antibody | anti-C1orf187 antibody

NEUCRIN Antibody

Cat. Num. AAA150960

• Gene Names: DRAXIN; UNQ3119; neucrin; AGPA3119; C1orf187
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NEUCRIN Antibody is affinity chromatography purified via peptide column.
• Synonyms: NEUCRIN; Polyclonal Antibody; NEUCRIN Antibody; UNQ3119; neucrin; AGPA3119; C1orf187; PSEC0258; UNQ3119/PRO10268; Draxin; Dorsal inhibitory axon guidance protein; chromosome 1 open reading frame 187; anti-C1orf187 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of Neucrin are known to exist; this antibody will detect the two shorter isoforms. Neucrin antibody is predicted to not cross-react with RILP.
• Purity/Purification: NEUCRIN Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 349

• Applicable Applications for anti-C1orf187 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Neucrin antibody can be used for detection of Neucrin by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Neucrin antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Neucrin.
• Buffer: NEUCRIN Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NEUCRIN antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Neucrin in rat cerebellum tissue lysate with Neucrin antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NEUCRIN in mouse brain tissue with NEUCRIN antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NEUCRIN in mouse brain tissue with NEUCRIN antibody at 20 μg/mL.)

Related Product Information for anti-C1orf187 antibody

NEUCRIN Antibody: Neucrin, also known as Draxin, is a repulsive guidance protein for the spinal cord and forebrain commissures. It is thought to act as an antagonist of canonical Wnt signaling by inhibiting the stabilization of cytosolic beta-catenin. Ectopically expressed neucrin inhibited growth or caused misrouting of chick spinal cord commissural axons in vivo while Neucrin-null mice showed defasciculation of spinal cord commissural axons and an absence of all forebrain commisures. Other experiments in mice have shown that olfactory bulb axonal outgrowth is inhibited by Neucrin, suggesting that Neucrin functions as an inhibitory guidance cue for olfactory bulb axons.

NCBI and Uniprot Product Information

• NCBI GI #: 86990448
• NCBI GeneID: 374946
• NCBI Accession #: BAG80561
• NCBI GenBank Nucleotide #: NM_198545.3
• UniProt Accession #: Q8NBI3; Q5SNX0; Q6UWK8; B6EV15
• Molecular Weight: 38,650 Da
• NCBI Official Full Name: draxin
• NCBI Official Synonym Full Names: dorsal inhibitory axon guidance protein
• NCBI Official Symbol: DRAXIN
• NCBI Official Synonym Symbols: UNQ3119; neucrin; AGPA3119; C1orf187
• NCBI Protein Information: draxin; dorsal repulsive axon guidance protein; dorsal inhibitory axon guidance protein; neural tissue-specific cysteine-rich protein
• UniProt Protein Name: Draxin
• UniProt Gene Name: DRAXIN
• UniProt Entry Name: DRAXI_HUMAN

Uniprot Description

DRAXIN: Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta- catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway. Belongs to the draxin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: extracellular region

Biological Process: dorsal spinal cord development; axon guidance; Wnt receptor signaling pathway; forebrain development; commissural neuron differentiation in the spinal cord; negative regulation of neuron apoptosis; negative regulation of axon extension