Carboxypeptidase Z Recombinant Protein | CPZ recombinant protein

Recombinant Carboxypeptidase Z (CPZ)

Cat. Num. AAA2028856

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Carboxypeptidase Z; Recombinant Carboxypeptidase Z (CPZ); CPZ recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MMDRSE NRCGGNFLKR GSIINGADWYSFTGGMSDFN YLHTNCFEIT VELGCVKFPP EEALYILWQH NKESLLNFVETVHRGIKGVV TDKFGKPVKN ARISVKGIRH DITTAPDGDY WRLLPPGIHIVIAQAPGYAK VIKKVIIPAR MKRAGRVDFI LQPLGMGPKN FIHGLRRTGPHDPLGGASSL GEATEPDPLR ARRQPSADGS KPWWWSYFTS LSTHRPRWLLKY
• Sequence Length: 641

• Applicable Applications for CPZ recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 29.4KDa
• Accurate Molecular Mass (KD): 29kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met425~Tyr652
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 2160714
• Molecular Weight: 58,591 Da
• NCBI Official Full Name: carboxypeptidase Z
• UniProt Protein Name: Carboxypeptidase Z
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPZ
• UniProt Synonym Gene Names: CPZ
• UniProt Entry Name: CBPZ_HUMAN

Uniprot Description

CPZ: Cleaves substrates with C-terminal arginine residues. Probably modulates the Wnt signaling pathway, by cleaving some undefined protein. May play a role in cleavage during prohormone processing. Belongs to the peptidase M14 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Secreted, signal peptide; Extracellular matrix; EC 3.4.17.-

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: Wnt receptor signaling pathway; proteolysis