Western Blot (WB)
(Anti-ATP1B3 rabbit polyclonal antibody at 1:500 dilutionLane A: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse ATP1B3 Polyclonal Antibody | anti-ATP1B3 antibody
Anti-ATP1B3 Antibody, Rabbit Polyclonal
Gene Names
Atp1b3; AA409958; AI664000; AW212096
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
ATP1B3; Polyclonal Antibody; Anti-ATP1B3 Antibody; Rabbit Polyclonal; Na; K-ATPase 3 subunit/ATP1B3 Antibody; Rabbit PAb; Antigen Affinity Purified; ATPase; Na+/K+ transporting; beta 3 polypeptide; Anti-AA409958 Antibody; Anti-AI664000 Antibody; Anti-AW212096 Antibody; anti-ATP1B3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Na, K-ATPase 3 subunit/ATP1B3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ATP1B3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:2500-1:10,000
IHC-P: 1:2500-1:10,000
Immunogen
A synthetic peptide corresponding to the center region of the Mouse Na, K-ATPase 3 subunit/ATP1B3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Na, K-ATPase 3 subunit/ATP1B3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ATP1B3 rabbit polyclonal antibody at 1:500 dilutionLane A: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ATP1B3 rabbit polyclonal antibody at 1:500 dilutionLane A: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of mouse ATP1B3 in mouse liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse ATP1B3 in mouse liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-ATP1B3 antibody
Enterovirus 71 (EV71) infection can cause severe diseases, and is becoming increasingly common in children. Human beta3 subunit of Na(+)/K(+)-ATPase (ATP1B3) protein was demonstrated to interact with the 3A protein of EV71. Although 3A protein had no effect on the expression of ATP1B3, EV71 infection resulted in elevated expression of ATP1B3 in RD cell line, both on messenger RNA (mRNA) and protein levels. And knockdown of ATP1B3 could significantly increase the replication of EV71, whereas overexpression of ATP1B3 significantly suppressed the replication of EV71 in RD cells. That the expression of ATP1B3 could induce the production of type-I interferons. ATP1B3 inhibit EV71 replication by enhancing the production of type-I interferons, which could act as a potential therapeutic target in EV71 infection.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,776 Da
NCBI Official Full Name
sodium/potassium-transporting ATPase subunit beta-3
NCBI Official Synonym Full Names
ATPase, Na+/K+ transporting, beta 3 polypeptide
NCBI Official Symbol
Atp1b3
NCBI Official Synonym Symbols
AA409958; AI664000; AW212096
NCBI Protein Information
sodium/potassium-transporting ATPase subunit beta-3
UniProt Protein Name
Sodium/potassium-transporting ATPase subunit beta-3
UniProt Gene Name
Atp1b3
UniProt Synonym Gene Names
ATPB-3
Uniprot Description
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Research Articles on ATP1B3
Similar Products
Product Notes
The ATP1B3 atp1b3 (Catalog #AAA8101896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ATP1B3 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATP1B3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 IHC-P: 1:2500-1:10,000. Researchers should empirically determine the suitability of the ATP1B3 atp1b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP1B3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
