Rabbit anti-Human MYL6B Polyclonal Antibody | anti-MYL6B antibody

Anti-MYL6B Antibody, Rabbit Polyclonal

Cat. Num. AAA8102061

• Gene Names: MYL6B; MLC1SA
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MYL6B; Polyclonal Antibody; Anti-MYL6B Antibody; Rabbit Polyclonal; MYL6B Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-MLC1SA Antibody; anti-MYL6B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MYL6B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MYL6B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human MYL6B
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MYL6B, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MYL6B rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:25 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-MYL6B antibody

MYL6B could facilitate the binding of MDM2 to p53, which consequently promotes the ubiquitination and degradation of p53 protein. MYL6B exerts the suppression effect on p53 as part of NMII holoenzymes because inhibiting the ATPase activity of myosin II heavy chain largely blocked this effect. MYL6B is overexpressed in HCC tissues and linked to the bad prognosis of HCC patients. Knocking out of MYL6B dramatically suppressed the clonogenic ability and increased the apoptosis level of HCC cell lines. MYL6B is a putative tumor driver gene in HCC which could promote the degradation of p53 by enhancing its' MDM2-mediated ubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 313851001
• NCBI GeneID: 140465
• NCBI Accession #: NP_001186558.1
• NCBI GenBank Nucleotide #: NM_001199629.1
• UniProt Accession #: P14649
• Molecular Weight: 22,764 Da
• NCBI Official Full Name: myosin light chain 6B
• NCBI Official Synonym Full Names: myosin, light chain 6B, alkali, smooth muscle and non-muscle
• NCBI Official Symbol: MYL6B
• NCBI Official Synonym Symbols: MLC1SA
• NCBI Protein Information: myosin light chain 6B; myosin alkali light chain 1 slow a; myosin light chain 1 slow a; myosin light chain 1 slow-twitch muscle A isoform; myosin light chain 1, slow-twitch muscle A isoform; myosin, light polypeptide 6B, alkali, smooth muscle and non-musc
• UniProt Protein Name: Myosin light chain 6B
• Protein Family: Myosin
• UniProt Gene Name: MYL6B
• UniProt Synonym Gene Names: MLC1SA; MLC1sa
• UniProt Entry Name: MYL6B_HUMAN

NCBI Description

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

MYL6B: Regulatory light chain of myosin. Does not bind calcium.

Protein type: Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: unconventional myosin complex; muscle myosin complex; myosin complex; cytosol

Molecular Function: protein binding; structural constituent of muscle; motor activity; calcium ion binding

Biological Process: skeletal muscle development; muscle contraction; metabolic process; muscle filament sliding