Typical Testing Data/Standard Curve
Human Olfactory receptor 14I1 (OR14I1) ELISA Kit | OR14I1 elisa kit
Human Olfactory receptor 14I1 (OR14I1) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||OR14I1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-OR14I1 antibody and an OR14I1-HRP conjugate. The assay sample and buffer are incubated together with OR14I1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OR14I1 concentration since OR14I1 from samples and OR14I1-HRP conjugate compete for the anti-OR14I1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OR14I1 from the sample, fewer sites are left to bind OR14I1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OR14I1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Uniprot Description
OR5BU1: Odorant receptor (Potential). Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q44
Cellular Component: plasma membrane; integral to membrane
Molecular Function: G-protein coupled receptor activity; olfactory receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; detection of chemical stimulus involved in sensory perception of smell
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Product Notes
The Human OR14I1 or14i1 (Catalog #AAA7218554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7218554 ELISA Kit recognizes Human OR14I1. It is sometimes possible for the material contained within the vial of "Olfactory receptor 14I1 (OR14I1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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