Rabbit anti-Human BVES Polyclonal Antibody | anti-BVES antibody

Anti-BVES Antibody, Rabbit Polyclonal

Cat. Num. AAA8101204

• Gene Names: BVES; POP1; HBVES; POPDC1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: BVES; Polyclonal Antibody; Anti-BVES Antibody; Rabbit Polyclonal; BVES/POPDC1 Antibody; Rabbit PAb; Antigen Affinity Purified; blood vessel epicardial substance; Anti-CARICK Antibody; Anti-HBVES Antibody; Anti-LGMD2X Antibody; Anti-POP1 Antibody; Anti-POPDC1 Antibody; anti-BVES antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BVES/POPDC1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BVES antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human BVES/POPDC1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human BVES/POPDC1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human BVES in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-BVES rabbit polyclonal antibody at 1:500 dilution.Lane A: Mosue skeletal muscle tissue lysateLane B: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB)

(Anti-BVES rabbit polyclonal antibody at 1:500 dilution Lane A: BVES konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:40 kDa (We are unsure as to the identity of these extra bands.))

Related Product Information for anti-BVES antibody

Blood vessel epicardial substance (BVES), or POPDC1, is a tight junction-associated transmembrane protein that modulates epithelial-to-mesenchymal transition (EMT) via junctional signaling pathways. BVES plays a protective role both in ulcerative and infectious colitis and identify BVES as a critical protector of colonic mucosal integrity. The Popeye domain containing1, also called Bves (Popdc1/Bves), is a transmembrane protein that functions in muscle regeneration, heart rate regulation, hypoxia tolerance, and ischemia preconditioning. The expression of Popdc1/Bves is elevated in cardiomyocytes maintained in serum free defined medium. Popdc1/Bves plays a role in the preservation of cardiomyocyte viability under serum deficiency through the alteration of Rac1 activity and the regulation of Bnip3 expression by FoxO3 and NFkappaB transcription factors pointing to Popdc1/Bves as a potential target to enhance heart protection. Blood vessel epicardial substance (BVES) is a tight junction-associated protein that regulates epithelial-mesenchymal states and is underexpressed in epithelial malignancy. Loss of BVES promotes inflammatory tumourigenesis through dysregulation of Wnt signalling and the oncogene c-Myc. BVES promoter methylation status may serve as a CAC biomarker. Blood vessel epicardial substance (BVES/Popdc1) is a junctional-associated transmembrane protein that is underexpressed in a number of malignancies and regulates epithelial-to-mesenchymal transition. BVES is a key regulator of intestinal stem cell programs and mucosal homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 38257661
• NCBI GeneID: 11149
• UniProt Accession #: Q8NE79; Q5T550; Q5T551; Q8IWC6; Q9HBV0; Q9UNG6; A8K1R4; E1P5D8
• Molecular Weight: 360
• NCBI Official Full Name: Blood vessel epicardial substance
• NCBI Official Synonym Full Names: blood vessel epicardial substance
• NCBI Official Symbol: BVES
• NCBI Official Synonym Symbols: POP1; HBVES; POPDC1
• NCBI Protein Information: blood vessel epicardial substance; popeye protein 1; popeye domain containing 1; popeye domain-containing protein 1
• UniProt Protein Name: Blood vessel epicardial substance
• Protein Family: Blood vessel epicardial substance
• UniProt Gene Name: BVES
• UniProt Synonym Gene Names: POP1; POPDC1; hBVES; Popeye protein 1
• UniProt Entry Name: POPD1_HUMAN

NCBI Description

This gene encodes a member of the POP family of proteins containing three putative transmembrane domains. This gene is expressed in cardiac and skeletal muscle and may play an important role in development of these tissues. The mouse ortholog may be involved in the regeneration of adult skeletal muscle and may act as a cell adhesion molecule in coronary vasculogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BVES: Cell adhesion molecule involved in the establishment and/or maintenance of cell integrity. Involved in the formation and regulation of the tight junction (TJ) paracellular permeability barrier in epithelial cells. Plays a role in VAMP3- mediated vesicular transport and recycling of different receptor molecules through its interaction with VAMP3. Plays a role in the regulation of cell shape and movement by modulating the Rho-family GTPase activity through its interaction with ARHGEF25/GEFT. Induces primordial adhesive contact and aggregation of epithelial cells in a Ca(2+)-independent manner. Also involved in striated muscle regeneration and repair and in the regulation of cell spreading. Belongs to the popeye family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: tight junction; plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: cAMP binding; structural molecule activity

Biological Process: vesicle-mediated transport; regulation of cell shape; regulation of membrane potential; muscle development; positive regulation of locomotion; regulation of heart rate; hemopoietic progenitor cell differentiation; positive regulation of receptor recycling