Typical Testing Data/Standard Curve (for reference only)
Rat Glutathione peroxidase 1 ELISA Kit | Gpx1 elisa kit
Rat Glutathione peroxidase 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPx-1, During the reaction, GPx-1 in the sample or standard competes with a fixed amount of biotin-labeled GPx-1 for sites on a pre-coated Monoclonal antibody specific to GPx-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GPx-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Jul 2016]
Uniprot Description
GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.
Protein type: EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Oxidoreductase
Chromosomal Location of Human Ortholog: 8q32
Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus
Molecular Function: glutathione peroxidase activity; SH3 domain binding
Biological Process: aging; angiogenesis involved in wound healing; apoptosis; blood vessel endothelial cell migration; cell proliferation; cell redox homeostasis; endothelial cell development; fat cell differentiation; glutathione metabolic process; heart contraction; hydrogen peroxide catabolic process; induction of apoptosis by oxidative stress; interaction with symbiont; lipid metabolic process; myoblast proliferation; myotube differentiation; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; protein amino acid oxidation; regulation of gene expression, epigenetic; regulation of mammary gland epithelial cell proliferation; regulation of neuron apoptosis; response to estradiol stimulus; response to folic acid; response to gamma radiation; response to glucose stimulus; response to hormone stimulus; response to hydrogen peroxide; response to hydroperoxide; response to nicotine; response to organic cyclic substance; response to oxidative stress; response to reactive oxygen species; response to selenium ion; response to symbiotic bacterium; response to toxin; response to wounding; response to xenobiotic stimulus; sensory perception of sound; skeletal muscle fiber development; skeletal muscle regeneration; thermoregulation; triacylglycerol metabolic process; UV protection; vasodilation
Research Articles on Gpx1
Similar Products
Product Notes
The Rat Gpx1 gpx1 (Catalog #AAA9425463) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9425463 ELISA Kit recognizes Rat Gpx1. It is sometimes possible for the material contained within the vial of "Glutathione peroxidase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
