Western Blot (WB)
(Anti-ARHGAP28 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human ARHGAP28 Polyclonal Antibody | anti-ARHGAP28 antibody
Anti-ARHGAP28 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
ARHGAP28; Polyclonal Antibody; Anti-ARHGAP28 Antibody; Rabbit Polyclonal; ARHGAP28 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-ARHGAP28 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ARHGAP28
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ARHGAP28 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:10,000-1:50,000
IHC-P: 1:10,000-1:50,000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human ARHGAP28
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ARHGAP28, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ARHGAP28 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ARHGAP28 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human ARGLU1 in human testis with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human ARGLU1 in human testis with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-ARHGAP28 antibody
ARHGAP28 is a GTPase-activating protein (GAP), and it converts activated RhoA to inactivated RhoA via GTPase. RhoA is a protein involved in cytoskeleton formation and cell motility. RhoA, for which ARHGAP28 acts as a GAP, is possibly a factor involved in the metastatic ability of cancer. An upregulation in RhoA expression was also detected suggesting that Arhgap28 may be able to additionally regulate Rho signaling at a transcriptional level.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
82,060 Da
NCBI Official Full Name
ARHGAP28 protein
NCBI Official Synonym Full Names
Rho GTPase activating protein 28
NCBI Official Symbol
ARHGAP28
NCBI Protein Information
rho GTPase-activating protein 28; rho-type GTPase-activating protein 28
UniProt Protein Name
Rho GTPase-activating protein 28
Protein Family
UniProt Gene Name
ARHGAP28
UniProt Synonym Gene Names
KIAA1314
UniProt Entry Name
RHG28_HUMAN
Uniprot Description
ARHGAP28: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 18p11.31
Cellular Component: nucleoplasm; cytosol; cell junction
Molecular Function: GTPase activator activity
Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity
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Product Notes
The ARHGAP28 arhgap28 (Catalog #AAA8102100) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARHGAP28 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 IHC-P: 1:10,000-1:50,000. Researchers should empirically determine the suitability of the ARHGAP28 arhgap28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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