Western Blot (WB)
(Anti-FSTL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse FSTL1 Polyclonal Antibody | anti-FSTL1 antibody
Anti-FSTL1 Antibody, Rabbit Polyclonal
Gene Names
Fstl1; Fstl; TSC-36; AI316791; AW107808
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
FSTL1; Polyclonal Antibody; Anti-FSTL1 Antibody; Rabbit Polyclonal; FSTL1 Antibody; Rabbit PAb; Antigen Affinity Purified; follistatin-like 1; Anti-AI316791 Antibody; Anti-AW107808 Antibody; Anti-Fstl Antibody; Anti-TSC-36 Antibody; anti-FSTL1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse FSTL1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FSTL1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse FSTL1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse FSTL1 (rM FSTL1). FSTL1 specific IgG was purified by Mouse FSTL1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-FSTL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-FSTL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-FSTL1 antibody
Follistatin-related protein 1 (FSTL1) is an extracellular glycoprotein whose functional significance in physiological and pathological processes is incompletely understood. Recently, we have shown that FSTL1 acts as a muscle-derived secreted factor that is up-regulated by Akt activation and ischemic stress and that FSTL1 exerts favorable actions on the heart and vasculature. Here, we sought to identify the receptor that mediates the cellular actions of FSTL1. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. FSTL1 is thought to be an autoantigen associated with rheumatoid arthritis. DIP2A functions as a novel receptor that mediates the cardiovascular protective effects of FSTL1. Experiment results have provided in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation, in part, through BMP4 signaling.
References
Rosenberg MI, et al. (2006) MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. J Cell Biol. 175(1): 77-85.Ouchi N, et al. (2010) DIP2A functions as a FSTL1 receptor. J Biol Chem. 285(10): 7127-34.Geng Y, et al. (2011) Follistatin-like 1 (Fstl1) is a bone morphogenetic protein (BMP) 4 signaling antagonist in controlling mouse lung development. Proc Natl Acad Sci U S A. 108(17): 7058-63.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,554 Da
NCBI Official Full Name
follistatin-related protein 1
NCBI Official Synonym Full Names
follistatin-like 1
NCBI Official Symbol
Fstl1
NCBI Official Synonym Symbols
Fstl; TSC-36; AI316791; AW107808
NCBI Protein Information
follistatin-related protein 1
UniProt Protein Name
Follistatin-related protein 1
Protein Family
UniProt Gene Name
Fstl1
UniProt Synonym Gene Names
Frp; Fstl; Tsc36
UniProt Entry Name
FSTL1_MOUSE
Uniprot Description
FSTL1: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: calcium ion binding; heparin binding; protein binding
Biological Process: response to starvation
Research Articles on FSTL1
Similar Products
Product Notes
The FSTL1 fstl1 (Catalog #AAA8112346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FSTL1 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FSTL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the FSTL1 fstl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FSTL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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