Immunohistochemistry (IHC)
(Immunochemical staining of human ACSS3 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human ACSS3 Polyclonal Antibody | anti-ACSS3 antibody
Anti-ACSS3 Antibody, Rabbit Polyclonal
Gene Names
ACSS3; FLJ21963
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
ACSS3; Polyclonal Antibody; Anti-ACSS3 Antibody; Rabbit Polyclonal; ACSS3 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-ACSS3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ACSS3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ACSS3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:2500-1:10,000
IP: 0.2-1uL/mg of lysate
IHC-P: 1:2500-1:10,000
IP: 0.2-1uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the center region of the Human ACSS3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ACSS3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human ACSS3 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human ACSS3 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(ACSS3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 1 uL anti-ACSS3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ACSS3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 75 kDa Observed band size: 75 kDa)
Immunoprecipitation (IP)
(ACSS3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 1 uL anti-ACSS3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ACSS3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 75 kDa Observed band size: 75 kDa)
Western Blot (WB)
(Anti-ACSS3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:75 kDa Observed band size:72 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ACSS3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:75 kDa Observed band size:72 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-ACSS3 antibody
Acetyl-CoA short chain synthetases (ACSSs) are key enzymes in the activation of fatty acids through the formation of thioesters with CoA. Three subfamily members are currently recognized in the human genome, ACSS1, ACSS2 and ACSS3, all single-copy genes. ACSS3 is poorly characterized. The acss3 transcripts are expressed in a wide range of tissues, with the highest levels observed in liver tissue followed by kidney tissue. Subcellular fractionation using liver tissue showed that ACSS3 is localized into the mitochondrial matrix. Advanced analyses found ACSS3 is prognosis biosignatures for multiple (gastric cancer) GCa disease conditions. While knockdown ACSS3 could suppress growth and invasion of GCa cells, of which even more impactful under starvation condition. Targeting ACSS3 could be a novel therapeutic strategy for cancer, in some case, GCa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,778 Da
NCBI Official Full Name
acyl-CoA synthetase short-chain family member 3, mitochondrial
NCBI Official Synonym Full Names
acyl-CoA synthetase short-chain family member 3
NCBI Official Symbol
ACSS3
NCBI Official Synonym Symbols
FLJ21963
NCBI Protein Information
acyl-CoA synthetase short-chain family member 3, mitochondrial; AMP-binding enzyme, 33217
UniProt Protein Name
Acyl-CoA synthetase short-chain family member 3, mitochondrial
Protein Family
UniProt Gene Name
ACSS3
UniProt Entry Name
ACSS3_HUMAN
Uniprot Description
ACSS3: Activates acetate so that it can be used for lipid synthesis or for energy generation. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.2.1.1; Ligase; Carbohydrate Metabolism - propanoate
Chromosomal Location of Human Ortholog: 12q21.31
Cellular Component: mitochondrion
Molecular Function: acetate-CoA ligase activity; ATP binding
Biological Process: metabolic process
Research Articles on ACSS3
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Product Notes
The ACSS3 acss3 (Catalog #AAA8102022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ACSS3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACSS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC-P: 1:2500-1:10,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the ACSS3 acss3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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