Western Blot (WB)
(Anti-CCL3 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:11 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Rat MIP-1 alpha/CCL3 Polyclonal Antibody | anti-CCL3 antibody
Anti-MIP-1 alpha/CCL3 Antibody, Rabbit Polyclonal
Gene Names
Ccl3; Scya3; MIP-1a
Reactivity
Rat
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MIP-1 alpha/CCL3; Polyclonal Antibody; Anti-MIP-1 alpha/CCL3 Antibody; Rabbit Polyclonal; CCL3/Mip1a Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-C motif) ligand 3; anti-CCL3 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat CCL3/Mip1a
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CCL3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Recombinant Rat CCL3/Mip1a Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat CCL3/Mip1a (rR CCL3/Mip1a). CCL3/Mip1a specific IgG was purified by Rat CCL3/Mip1a affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CCL3 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:11 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CCL3 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:11 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-CCL3 antibody
CCL3 is a cytokine belonging to the CC chemokine family. Chemokines are a family of structurally related leukocyte chemoattractant cytokines that play a central role during immunoregulatory and inflammation processes. All chemokines contain four conserved cysteines linked by disulfide bonds, and two major subfamilies, namely CXC and CC, are defined on the basis of the first two cysteines which are separated by one amino acid or are adjacent. CCL3 is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes.
Product Categories/Family for anti-CCL3 antibody
References
Zhao RY, et al. (2005) Viral infections and cell cycle G2/M regulation. Cell Res. 15(3):143-9. Joseph AM, et al. (2005) Nef: "necessary and enforcing factor" in HIV infection. Curr HIV Res. 3(1):87-94. Muthumani K, et al. (2004) HIV-1 Vpr and anti-inflammatory activity. DNA Cell Biol. 23(4): 239-47.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,335 Da
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
Ccl3
NCBI Official Synonym Symbols
Scya3; MIP-1a
NCBI Protein Information
C-C motif chemokine 3; MIP-1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; small inducible cytokine A3; small-inducible cytokine A3
UniProt Protein Name
C-C motif chemokine 3
Protein Family
UniProt Gene Name
Ccl3
UniProt Synonym Gene Names
Mip1a; Scya3; MIP-1-alpha
UniProt Entry Name
CCL3_RAT
NCBI Description
mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]
Uniprot Description
Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
Research Articles on CCL3
Similar Products
Product Notes
The CCL3 ccl3 (Catalog #AAA8112698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MIP-1 alpha/CCL3 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MIP-1 alpha/CCL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CCL3 ccl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIP-1 alpha/CCL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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