SDS-Page
(Detection of AP-2β. 30ug of whole cell extracts from exponentially growing human SHsy5y cells, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with 1.0ug/ml goat anti-AP-2β antibody, as described in Protocols for Immunoblotting.)
Goat anti-Human Activating enhancer-binding protein 2-beta Polyclonal Antibody
Activating enhancer-binding protein 2-beta (AP-2 beta, TFAP2B)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Activating enhancer-binding protein 2-beta; Polyclonal Antibody; Activating enhancer-binding protein 2-beta (AP-2 beta; TFAP2B); Anti -Activating enhancer-binding protein 2-beta (AP-2 beta; anti-Activating enhancer-binding protein 2-beta antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human AP-2 beta at ~60kD.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Applicable Applications for anti-Activating enhancer-binding protein 2-beta antibody
Western Blot (WB)
Application Notes
Dilution: Western Blot: 1.0ug/ml
Immunogen
Recombinant human AP-2 beta, aa121-218; Q92481 (E. coli).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of AP-2β. 30ug of whole cell extracts from exponentially growing human SHsy5y cells, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with 1.0ug/ml goat anti-AP-2β antibody, as described in Protocols for Immunoblotting.)
SDS-Page
(Detection of AP-2β. 30ug of whole cell extracts from exponentially growing human SHsy5y cells, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with 1.0ug/ml goat anti-AP-2β antibody, as described in Protocols for Immunoblotting.)
Related Product Information for anti-Activating enhancer-binding protein 2-beta antibody
AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52kD member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460aa in length. It contains a transactivation domain (aa41-131), a DNA-binding motif (aa132-298) and a C-terminal dimerization region (aa299-439). SUMOylation occurs on Lys21, adding 15kD of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development.
Product Categories/Family for anti-Activating enhancer-binding protein 2-beta antibody
Similar Products
Product Notes
The Activating enhancer-binding protein 2-beta (Catalog #AAA623166) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Activating enhancer-binding protein 2-beta (AP-2 beta, TFAP2B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Activating enhancer-binding protein 2-beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Dilution: Western Blot: 1.0ug/ml. Researchers should empirically determine the suitability of the Activating enhancer-binding protein 2-beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Activating enhancer-binding protein 2-beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
