Rabbit AP-2 beta Polyclonal Antibody | anti-TFAP2B antibody

AP-2 beta

Cat. Num. AAA621420

• Gene Names: TFAP2B; AP-2B; AP2-B; MGC21381; TFAP2B
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: AP-2 beta; Polyclonal Antibody; Anti -AP-2 beta; anti-TFAP2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total human AP-2 beta protein at 49kD. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-TFAP2B antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.; Dilution: Western Blot 1:1000; Immunoprecipitation 1:100; Immunofluorescence (IF-IC) 1:100
• Immunogen: Synthetic peptide derived from the sequence of human AP-2 beta (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts using MBS621420.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SH-SY5Y cells labeled with MBS621420 (green, left) compared to a nonspecific negative control antibody (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)

Related Product Information for anti-TFAP2B antibody

The sequence-specific transcription factor activator protein 2 beta (AP-2 beta) is required for normal kidney development (1). AP-2 beta knockout mice die 1-2 days after birth due to polycystic kidney disease (1). Massive apoptosis occured during kidney deveopment at the end of embryogenesis in these mice (1). Overexpressed AP-2 beta has been to found to suppress c-myc-induced apoptosis, indicating a role of this transcription factor in cell survival (1). In addition, overexpression of AP-2 beta is shown to be related to impaired insulin signaling in adipocytes, and is therefore proposed to be a candidate gene that may relate to obesity (2).

Product Categories/Family for anti-TFAP2B antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1495417
• NCBI GeneID: 7021
• UniProt Accession #: Q92481; Q5JYX6; Q9NQ63; Q9NU99; Q9UJI7; Q9Y214; Q9Y3K3
• Molecular Weight: 50,474 Da
• NCBI Official Full Name: AP-2 beta
• NCBI Official Synonym Full Names: transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
• NCBI Official Symbol: TFAP2B
• NCBI Official Synonym Symbols: AP-2B; AP2-B; MGC21381; TFAP2B
• NCBI Protein Information: transcription factor AP-2-beta; AP2-beta; activating enhancer binding protein 2 beta; activating enhancer-binding protein 2-beta
• UniProt Protein Name: Transcription factor AP-2-beta
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP2B
• UniProt Entry Name: AP2B_HUMAN

NCBI Description

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq]

Uniprot Description

AP-2 beta: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity. Belongs to the AP-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; caspase inhibitor activity; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: skin development; fat cell differentiation; transcription from RNA polymerase II promoter; hindlimb morphogenesis; response to lithium ion; positive regulation of transcription, DNA-dependent; regulation of BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; glucose homeostasis; calcium ion homeostasis; forelimb morphogenesis; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; regulation of cell differentiation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; sodium ion homeostasis; phosphate ion homeostasis; negative regulation of neuron apoptosis; kidney development; potassium ion homeostasis; response to drug; glucose metabolic process; renal water homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; negative regulation of apoptosis

Disease: Char Syndrome