Typical Testing Data/Standard Curve (for reference only)
Human High Mobility Group Protein B1 (HMGB1) ELISA Kit | HMGB1 elisa kit
High Mobility Group Protein B1 (HMGB1) ELISA
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human
Synonyms
High Mobility Group Protein B1 (HMGB1); High Mobility Group Protein B1 (HMGB1) ELISA; HMGB1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of HMGB1. No significant cross-reactivity or interference between HMGB1 and analogues was observed.
Sequence Length
215
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Sandwich
Detection Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HMGB1 elisa kit
Intended Uses: The Human High mobility group protein B1 (HMGB1) ELISA has been designed for the detection of HMGB1 in serum, plasma, tissue homogenates and other biological fluids. For research use only,
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody is pre-coated onto 96-well plates. And the biotin conjugated antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody is pre-coated onto 96-well plates. And the biotin conjugated antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
high mobility group protein B1 isoform 1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; HMG-1; SBP-1
NCBI Protein Information
high mobility group protein B1
Protein Family
NCBI Description
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Research Articles on HMGB1
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Product Notes
The Human HMGB1 (Catalog #AAA495584) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495584 ELISA Kit recognizes Human HMGB1. It is sometimes possible for the material contained within the vial of "High Mobility Group Protein B1 (HMGB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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