Histone H3 Peptide

Histone H3, phosphorylated (Ser10) Blocking Peptide

Cat. Num. AAA659312

• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified
• Synonyms: Histone H3; phosphorylated (Ser10) Blocking Peptide; Histone H3 peptide

• Specificity: Note: Adherence to the attached protocol is extremely important.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a lyophilized powder.

• Applicable Applications for Histone H3 peptide: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of Phospho-Histone H3 (Ser10) in control and serum plus calyculin A treated NIH/3T3 cell extracts, using Phospho-Histone H3 (Ser10) Antibody alone (left) and the same antibody preincubated with Phospho-Histone H3 (Ser10) Blocking Peptide (right).)

Related Product Information for Histone H3 peptide

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation (2-4). These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6).

Synthetic phosphopeptide corresponding to residues surrounding serine 10 of human histone H3.
Corresponding antibody, H5110-13J, detects endogenous histone H3 only when phosphorylated at serine 10. It is a useful tool in characterizing histone phosphorylation and in monitoring mitosis and meiosis with immunocytochemistry. Does not crossreact with other phosphorylated histones, or with histone H3 modified by acetylation. Recognizes human, mouse, rat, monkey.

Product Categories/Family for Histone H3 peptide

Molecular Biology; MB-Histones

NCBI and Uniprot Product Information

• NCBI GI #: 3273350
• NCBI Official Full Name: histone H3
• Protein Family: Histone

Uniprot Description

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.