Western Blot (WB)
(Western blot analysis of lysates from HeLa and NIH/3T3 cells, untreated or TSA-treated (400nM for 18 hours) using MBS602713.)
Rabbit anti-Human, Mouse Histone H3, acetylated Monoclonal Antibody | anti-H3 antibody
Histone H3, acetylated (Lys9)
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
SupernatantSupernatant
Synonyms
Histone H3; acetylated; Monoclonal Antibody; acetylated (Lys9); Anti -Histone H3; anti-H3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
7H26
Specificity
Recognizes endogenous levels of human histone H3 at ~17kD only when acetylated on Lys9. Does not crossreact with other acetylated histones. Species Crossreactivity: mouse, rat, monkey, zebrafish.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.15M sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-H3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Flow Cytometry.
Dilution: Western Blot: 1:1000
Chromatin Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:400
Immunohistochemistry (paraffin): 1:800. Recommend antigen retrieval by heating in citrate buffer.
Flow Cytometry: 1:200
Dilution: Western Blot: 1:1000
Chromatin Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:400
Immunohistochemistry (paraffin): 1:800. Recommend antigen retrieval by heating in citrate buffer.
Flow Cytometry: 1:200
Immunogen
Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of lysates from HeLa and NIH/3T3 cells, untreated or TSA-treated (400nM for 18 hours) using MBS602713.)
Western Blot (WB)
(Western blot analysis of lysates from HeLa and NIH/3T3 cells, untreated or TSA-treated (400nM for 18 hours) using MBS602713.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of untreated HeLa cells using MBS602713 versus propidium iodide (DNA content). Note positive staining in cycling cells (box).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of untreated HeLa cells using MBS602713 versus propidium iodide (DNA content). Note positive staining in cycling cells (box).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TSA-treated (right), using MBS602713 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TSA-treated (right), using MBS602713 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)
Related Product Information for anti-H3 antibody
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4) is the primary building block of chromatin (1). The amino- terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methyl- ation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23 (2,3). Acetyl- ation at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Staining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
NCBI Protein Information
histone H3
Protein Family
Research Articles on H3
Similar Products
Product Notes
The H3 (Catalog #AAA602713) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, acetylated (Lys9) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, acetylated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Flow Cytometry. Dilution: Western Blot: 1:1000 Chromatin Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:400 Immunohistochemistry (paraffin): 1:800. Recommend antigen retrieval by heating in citrate buffer. Flow Cytometry: 1:200. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, acetylated, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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