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Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells, untreated or TSA-treated, using Acetyl-Histone H3 (Lys18) Antibody.)
Rabbit Histone H3, acetylated Polyclonal Antibody | anti-H3 antibody
Histone H3, acetylated (Lys18)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A and immunoaffinity chromatography.
Synonyms
Histone H3; acetylated; Polyclonal Antibody; acetylated (Lys18); Anti -Histone H3; anti-H3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of human Lys18 acetylated histone H3
Purity/Purification
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Purified by Protein A and immunoaffinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-H3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (ABC): 1:50
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (ABC): 1:50
Immunogen
Synthetic acetyl-Lys18 peptide corresponding to residues surrounding Lys18 of human histone H3 (KLH coupled).
Note
Adherence to the attached protocol is extremely important.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells, untreated or TSA-treated, using Acetyl-Histone H3 (Lys18) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells, untreated or TSA-treated, using Acetyl-Histone H3 (Lys18) Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder, showing nuclear localization, using Acetyl-Histone H3 (Lys18) Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder, showing nuclear localization, using Acetyl-Histone H3 (Lys18) Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin embedded NIH/3T3 cells, untreated (left) or TSA-treated (right), using Acetyl-Histone H3 (Lys18) Antibody. (no counterstain))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin embedded NIH/3T3 cells, untreated (left) or TSA-treated (right), using Acetyl-Histone H3 (Lys18) Antibody. (no counterstain))
Immunocytochemistry (ICC)
(Immunocytochemical staining of HeLa cells, untreated (left) or TSA-treated (400 nM for 12 hours) (right), using Acetyl-Histone H3 (Lys18) Antibody.)
Immunocytochemistry (ICC)
(Immunocytochemical staining of HeLa cells, untreated (left) or TSA-treated (400 nM for 12 hours) (right), using Acetyl-Histone H3 (Lys18) Antibody.)
Related Product Information for anti-H3 antibody
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
NCBI Protein Information
histone H3
Protein Family
Research Articles on H3
Similar Products
Product Notes
The H3 (Catalog #AAA613901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, acetylated (Lys18) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, acetylated can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50 Immunocytochemistry (ABC): 1:50. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, acetylated, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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