Testing Data
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Histone H3 Protein
Histone H3 (F3), Arginine Rich
Purity
Purified
Synonyms
Histone H3; Histone H3 (F3); Arginine Rich; Histone H3 protein
Host
Bovine tissue (thymus)
Specificity
Bands at 15.3, 14 kD (20%SDS-PAGE)
Purity/Purification
Purified
Form/Format
Supplied as a white powder. Reconstitute with cold, sterile 0.9M acetic acid to ~1mg/ml. Prepare fresh solution before use.
Country of Origin
USA
Preparation and Storage
Store lyophilized histones desiccated at 4 degree C. Reconstitute per reconstitution instructions. Do not store reconstituted histones. Histones lose activity in solutions. Further dilutions can be made in assay buffer.
Testing Data
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Testing Data
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Related Product Information for Histone H3 protein
Histone subclass H3(F3), may also contain H2b(F2b) and H2a(F2a2).
Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. ThiS allows for the supercoiling of the chromatin fibers. With the exception of H 1 , the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly - conserved (low mutation rate) proteins in nature. In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The hlstones are a group of water and dilute acid soluble l5asic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limitednumber of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamiC such that the histones of a single cell type are found to vary during development. They are believed to playa role in gene activity and cellular metabolism.
Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. ThiS allows for the supercoiling of the chromatin fibers. With the exception of H 1 , the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly - conserved (low mutation rate) proteins in nature. In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The hlstones are a group of water and dilute acid soluble l5asic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limitednumber of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamiC such that the histones of a single cell type are found to vary during development. They are believed to playa role in gene activity and cellular metabolism.
Product Categories/Family for Histone H3 protein
NCBI and Uniprot Product Information
Uniprot Description
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Product Notes
The Histone H3 (Catalog #AAA634302) is a Protein produced from Bovine tissue (thymus) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Histone H3, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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