Rabbit Caspase-6 Monoclonal Antibody | anti-CASP6 antibody

Caspase-6 Antibody

Cat. Num. AAA9435736

• Gene Names: CASP6; MCH2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Caspase-6; Monoclonal Antibody; Caspase-6 Antibody; Apoptotic protease Mch-2 antibody; Apoptotic protease MCH2 antibody; CASP-6 antibody; CASP6 antibody; CASP6_HUMAN antibody; Caspase 6 antibody; Caspase 6 apoptosis related cysteine protease antibody; Caspase 6; apoptosis related cysteine peptidase antibody; Caspase-6 subunit p11 antibody; Mch2 antibody; OTTHUMP00000162742 antibody; OTTHUMP00000162743 antibody; anti-CASP6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: SC56-09
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-CASP6 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Caspase-6 on different lysates using anti-Caspase-6 antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse colon Lane 2: MCF-7)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Caspase-6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-CASP6 antibody

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/ caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly (ADP-ribose)polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

Product Categories/Family for anti-CASP6 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 14916483
• NCBI GeneID: 839
• NCBI Accession #: NP_001217.2
• NCBI GenBank Nucleotide #: NM_001226.3
• UniProt Accession #: P55212; Q9BQE7
• Molecular Weight: 33,310 Da
• NCBI Official Full Name: caspase-6 isoform alpha preproprotein
• NCBI Official Synonym Full Names: caspase 6, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP6
• NCBI Official Synonym Symbols: MCH2
• NCBI Protein Information: caspase-6; apoptotic protease MCH-2; caspase 6, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-6
• Protein Family: Caspase
• UniProt Gene Name: CASP6
• UniProt Synonym Gene Names: MCH2; CASP-6
• UniProt Entry Name: CASP6_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.59; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: epithelial cell differentiation; apoptosis; proteolysis; cell structure disassembly during apoptosis