Typical Testing Data/Standard Curve (for reference only)
Human Alpha endosulfine (ENSA) ELISA Kit | ENSA elisa kit
Human Alpha endosulfine (ENSA) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ENSA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ENSA antibody and an ENSA-HRP conjugate. The assay sample and buffer are incubated together with ENSA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ENSA concentration since ENSA from samples and ENSA-HRP conjugate compete for the anti-ENSA antibody binding site. Since the number of sites is limited, as more sites are occupied by ENSA from the sample, fewer sites are left to bind ENSA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ENSA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
ENSA: a cytoplasmic, highly conserved cAMP-regulated phosphoprotein (ARPP) and regulator of ATP-sensitive potassium (KATP) channels. An endogenous ligand for the sulfonylurea receptor, ABCC8, the regulatory subunit of the KATP channel located on the plasma membrane of pancreatic beta cells. Modulates insulin secretion through the interaction with ABCC8, and may be associated with type 2 diabetes. Eight alternatively spliced isoforms have been observed.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: phosphatase inhibitor activity; protein phosphatase 2A binding; protein phosphatase type 2A regulator activity; protein phosphatase inhibitor activity; potassium channel inhibitor activity; ion channel inhibitor activity; receptor binding
Biological Process: mitosis; transport; cell division; regulation of catalytic activity; negative regulation of catalytic activity; response to glucose stimulus; mitotic cell cycle; G2/M transition of mitotic cell cycle; regulation of insulin secretion; response to nutrient
Research Articles on ENSA
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Product Notes
The Human ENSA ensa (Catalog #AAA7221926) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7221926 ELISA Kit recognizes Human ENSA. It is sometimes possible for the material contained within the vial of "Alpha endosulfine (ENSA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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