Mouse Histone H3 Monoclonal Antibody | anti-H3 antibody

Histone H3, phosphorylated (Ser10)

Cat. Num. AAA633373

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Ascites; Ascites.
• Synonyms: Histone H3; Monoclonal Antibody; phosphorylated (Ser10); Anti -Histone H3; anti-H3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A50
• Specificity: Recognizes human histone H3 only when phosphorylated at Ser10 at 17kD. Does not cross-react with other phosphorylatied histones or acetylated of histone H3. Species Cross-reactivity: Mouse, rat, Drosophila.
• Purity/Purification: Ascites; Ascites.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-H3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot,, Immunofluorescence, Immunocytochemistry and Flow Cytometry.; Dilution: Immunofluorescence(IF-IC): 1:100; Immunofluorescence(IF-F): 1:100; Flow Cytometry: 1:25; Western Blot: 1:1000 Incubate membrane with diluted antibody in 1X TBS, 0.1% Tween-20, 5% BSA at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic phosphopeptide corresponding to residues around Ser10 of human histone H3 (KLH).
• Note: Adherence to the attached protocol is extremely important.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of whole cell lysates from NIH/3T3 cells, untreated or treated with serum plus calyculin A (to induce phosphorylation of H3), using MBS633373 (top), H5110-13J (middle) or Histone H3 Antibody (bottom).)

Testing Data

(Mitotic-specific staining of third instar wild type Drosophila larval neuroblasts, using MBS633373. Images show late prophase (A), metaphase (B) and anaphase (C, D). (provided by Marie-Louise Loupart, University of Edinburgh Scotland.))

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MBS633373. Staining of untreated (blue) or serum/calyculin-treated (green) Ramos cells compared to a nonspecific negative control antibody (red).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of proliferating/mitotic cells labeled with MBS633373 (blue) in the subventricular zone following 4 hr reperfusion after cerebral ischemia. Red = EGR1 antibody. Green = Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate).)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of pacilitaxel-treated THP-1 cells, using MBS633373 vs propidium iodide (DNA content). Red population indicates positive Phospho-Histone H3 cells.)

Related Product Information for anti-H3 antibody

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation (2-4). These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6).

Product Categories/Family for anti-H3 antibody

Antibodies; Abs to Histones

NCBI and Uniprot Product Information

• NCBI GI #: 47551065
• NCBI GeneID: 373335
• NCBI Accession #: NP_999709.1
• NCBI GenBank Nucleotide #: NM_214544.1
• NCBI Official Full Name: histone H3
• NCBI Official Symbol: H3
• NCBI Protein Information: histone H3
• Protein Family: Histone