Mouse anti-Human SPANXB1 Polyclonal Antibody | anti-SPANXB1 antibody

SPANXB1 (Sperm Protein Associated with the Nucleus on the X Chromosome B/F, SPANX Family Member B/F, Cancer/testis Antigen 11.2, CT11.2, Nuclear-associated Protein SPAN-Xb, SPANXB, SPANX-B, SPANXB2, Nuclear-associated Protein SPAN-Xf, SPANX-F, SPANXF1)

Cat. Num. AAA6004689

• Gene Names: SPANXB1; B1; CT11.2; SPANXB; SPANX-B
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SPANXB1; Polyclonal Antibody; SPANXB1 (Sperm Protein Associated with the Nucleus on the X Chromosome B/F; SPANX Family Member B/F; Cancer/testis Antigen 11.2; CT11.2; Nuclear-associated Protein SPAN-Xb; SPANXB; SPANX-B; SPANXB2; Nuclear-associated Protein SPAN-Xf; SPANX-F; SPANXF1); Anti -SPANXB1 (Sperm Protein Associated with the Nucleus on the X Chromosome B/F; anti-SPANXB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SPANXB1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MGQQSSVRRLKRSVPCESNEANEANEANKTMPETPTGDSDPQPAPKKMKTSESSTILVVRYRRNVKRTSPEELVNDHARENRINPDQMEEEEFIEITTERPKK

• Applicable Applications for anti-SPANXB1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human SPANXB1, aa1-103 (NP_115850.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate (11.33kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to SPANXB1 on Hs 181.Tes cell. [antibody concentration 10ug/ml].)

Product Categories/Family for anti-SPANXB1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 14196344
• NCBI GeneID: 728695
• NCBI Accession #: NP_115850.1
• NCBI GenBank Nucleotide #: NM_032461.2
• UniProt Accession #: Q9NS25; Q32WQ7; Q5JYZ7; Q8TAD5; B2RPP2
• Molecular Weight: 11,826 Da
• NCBI Official Full Name: sperm protein associated with the nucleus on the X chromosome B/F
• NCBI Official Synonym Full Names: SPANX family, member B1
• NCBI Official Symbol: SPANXB1
• NCBI Official Synonym Symbols: B1; CT11.2; SPANXB; SPANX-B
• NCBI Protein Information: sperm protein associated with the nucleus on the X chromosome B/F; SPANX family member B/F; cancer/testis antigen 11.2; nuclear-associated protein SPAN-Xb; nuclear-associated protein SPAN-Xf; cancer/testis antigen family 11, member 2; sperm protein associated with the nucleus, X chromosome, family member B1
• UniProt Protein Name: Sperm protein associated with the nucleus on the X chromosome B/F
• Protein Family: Sperm protein associated with the nucleus on the X chromosome
• UniProt Gene Name: SPANXB1
• UniProt Synonym Gene Names: SPANXB; CT11.2; SPANX-B; SPANX-F
• UniProt Entry Name: SPNXB_HUMAN

NCBI Description

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

SPANXB1: Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: cytoplasm; nucleus

Biological Process: spermatid development