SDS-Page
(AbFlex Supt5H/SPT5 antibody tested by Western blot. 20 ug of HeLa whole cell extract was run on SDS-PAGE and probed with AbFlex Supt5H/SPT5 antibody at 2 ug/ml.)
Mouse anti-Human Supt5H/SPT5 Antibody | anti-Supt5H/SPT5 antibody
AbFlex Supt5H/SPT5 antibody (rAb)
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
Supt5H/SPT5; AbFlex Supt5H/SPT5 antibody (rAb); SPT5 Homolog; DSIF Elongation Factor Subunit; DSIF Large Subunit; Transcription Elongation Factor SPT5; anti-Supt5H/SPT5 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Supt5H/SPT5 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.5-2ug/ml
IP: 4ug per IP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
WB: 0.5-2ug/ml
IP: 4ug per IP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a synthetic peptide derived from the human SPT5 C-terminal region.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex Supt5H/SPT5 antibody tested by Western blot. 20 ug of HeLa whole cell extract was run on SDS-PAGE and probed with AbFlex Supt5H/SPT5 antibody at 2 ug/ml.)
SDS-Page
(AbFlex Supt5H/SPT5 antibody tested by Western blot. 20 ug of HeLa whole cell extract was run on SDS-PAGE and probed with AbFlex Supt5H/SPT5 antibody at 2 ug/ml.)
Western Blot (WB)
(AbFlex Supt5H/SPT5 antibody tested by Immunoprecipitation. 4 ug of antibody was used to immunoprecipitate Supt5H/SPT5 from 500 ug of HeLa whole cell extract, which was then run on SDS-PAGE and probed with the same antibody at 2 u/ml. Tween-20 at 0.05% was added to blocking buffers to perform Western blot and reduce background.)
Western Blot (WB)
(AbFlex Supt5H/SPT5 antibody tested by Immunoprecipitation. 4 ug of antibody was used to immunoprecipitate Supt5H/SPT5 from 500 ug of HeLa whole cell extract, which was then run on SDS-PAGE and probed with the same antibody at 2 u/ml. Tween-20 at 0.05% was added to blocking buffers to perform Western blot and reduce background.)
Related Product Information for anti-Supt5H/SPT5 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Supt5H/SPT5 antibody (rAb) was raised in a Mouse host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Supt5H/SPT5 was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Supt5H/SPT5 was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.
Product Categories/Family for anti-Supt5H/SPT5 antibody
NCBI and Uniprot Product Information
Molecular Weight
140kDa
Similar Products
Product Notes
The Supt5H/SPT5 (Catalog #AAA389520) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Supt5H/SPT5 antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Supt5H/SPT5 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml IP: 4ug per IP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the Supt5H/SPT5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Supt5H/SPT5, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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