IkappaB-alpha Antibody | anti-NFKBIA antibody

IkappaB-alpha (Ab-32/36) Antibody

Cat. Num. AAA8510174

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: IkappaB-alpha; IkappaB-alpha (Ab-32/36) Antibody; anti-NFKBIA antibody

• Reactivity: Human, Mouse, Rat
• Specificity: IkappaB-alpha (Ab-32/36) Antibody detects endogenous levels of total IkappaB-alpha protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 317

• Applicable Applications for anti-NFKBIA antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human IkappaB-alpha around the phosphorylation site of Serine 32/36.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded breast carcinoma, using IkappaB-alpha (Ab-32/36) Antibody. Left: Untreated; Right: Treated with synthesized peptide.)

Western Blot (WB)

(Western blot analysis of extracts from MCF7 cells treated with TNF-alpha: Line1: Using IkappaB-alpha (Ab-32/36) Antibody ; Line2: The same antibody pre-incubated with synthesized peptide.)

References

Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44. Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15. Nair A, et al. (2003) Oncogene; 22(1): 50-8. Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53. Schubert SY, et al. (2002)FASEB J; 16(14): 1931-3.

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35,609 Da
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: NFKB inhibitor alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.