Rabbit SSR3 Polyclonal Antibody | anti-SSR3 antibody

Anti-SSR3 antibody

Cat. Num. AAA175421

• Gene Names: SSR3; TRAPG
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: SSR3; Polyclonal Antibody; Anti-SSR3 antibody; Translocon-associated protein subunit gamma(TRAP-gamma); signal sequence receptor; gamma(translocon-associated protein gamma); Signal sequence receptor subunit gamma antibody; SSR-gamma antibody; Ssr3 antibody; SSRG_HUMAN antibody; Translocon-associated protein subunit gamma antibody; TRAP-gamma antibody; TRAPG antibody; anti-SSR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 185

• Applicable Applications for anti-SSR3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3 (8-23aa KQQSEEDLLLQDFSRN), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-SSR3 antibody, MBS175421, Western blottingAll lanes: Anti SSR3 (MBS175421) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: A431 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 21KDObserved bind size: 21KD)

Immunohistochemistry (IHC)

(Anti-SSR3 antibody, MBS175421, IHC(P)IHC(P): Human Prostatic Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-SSR3 antibody, MBS175421, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-SSR3 antibody, MBS175421, IHC(P)IHC(P): Rat Intestine Tissue )

Related Product Information for anti-SSR3 antibody

Description: Rabbit IgG polyclonal antibody for Translocon-associated protein subunit gamma(SSR3) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: SSR3(signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al.(1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross(2012)mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence(GRCh37). Hartmann et al.(1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.

NCBI and Uniprot Product Information

• NCBI GI #: 9087205
• NCBI GeneID: 6747
• UniProt Accession #: Q9UNL2; Q549M4; B2R7D0; B4E2P2; D3DNK5
• Molecular Weight: 22,610 Da
• NCBI Official Full Name: Translocon-associated protein subunit gamma
• NCBI Official Synonym Full Names: signal sequence receptor, gamma (translocon-associated protein gamma)
• NCBI Official Symbol: SSR3
• NCBI Official Synonym Symbols: TRAPG
• NCBI Protein Information: translocon-associated protein subunit gamma; SSR gamma; SSR-gamma; TRAP-gamma; TRAP-complex gamma subunit; signal sequence receptor subunit gamma; translocon-associated protein gamma subunit
• UniProt Protein Name: Translocon-associated protein subunit gamma
• Protein Family: SWI/SNF and RSC complexes
• UniProt Gene Name: SSR3
• UniProt Synonym Gene Names: TRAPG; TRAP-gamma; SSR-gamma
• UniProt Entry Name: SSRG_HUMAN

NCBI Description

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR is comprised of four membrane proteins/subunits: alpha, beta, gamma, and delta. The first two are glycosylated subunits and the latter two are non-glycosylated subunits. This gene encodes the gamma subunit, which is predicted to span the membrane four times. [provided by RefSeq, Aug 2010]

Uniprot Description

SSR3: TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. Belongs to the TRAP-gamma family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q25.31

Cellular Component: integral to endoplasmic reticulum membrane; translocon complex

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; gene expression