Chromatin Immunoprecipitation (ChIP)
(AbFlex AM-Tag antibody (rAb) tested by ChIP. ’s pAM_1C_JunD Vector was transiently transfected or mock transfected into HCT116 cells. Chromatin was harvested according to the instructions in the Tag-ChIP-IT Kit . 10 ug of the AM-Tag antibody was used to immunoprecipitate the cross-linked AM-Tag-JunD fusion protein. qPCR data shows enrichment of AM-Tag-JunD with the FOXRED qPCR primer set and little to no enrichment in the mock transfections or when using the negative control Unt12 qPCR primer set.)
Mouse anti-Human AM Antibody | anti-AM antibody
AbFlex AM-Tag antibody (rAb)
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, ELISA, Western Blot
Purity
Protein A Chromatography
Synonyms
AM; AbFlex AM-Tag antibody (rAb); anti-AM antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 200mM Hepes pH7.5, 100mM NaCl, 50mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-AM antibody
Chromatin Immunoprecipitation (ChIP), ELISA (EIA), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 10ug per ChIP
WB: 0.5-2ug/ml dilution
Bead-based ELISA: 7-560 ng/ml
ChIP: 10ug per ChIP
WB: 0.5-2ug/ml dilution
Bead-based ELISA: 7-560 ng/ml
Antibody Type
Recombinant Antibody
Tag
AM-Tag
Immunogen
This antibody was raised against Our unique AM-Tag sequence for use with Tag-ChIP-IT analysis.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(AbFlex AM-Tag antibody (rAb) tested by ChIP. ’s pAM_1C_JunD Vector was transiently transfected or mock transfected into HCT116 cells. Chromatin was harvested according to the instructions in the Tag-ChIP-IT Kit . 10 ug of the AM-Tag antibody was used to immunoprecipitate the cross-linked AM-Tag-JunD fusion protein. qPCR data shows enrichment of AM-Tag-JunD with the FOXRED qPCR primer set and little to no enrichment in the mock transfections or when using the negative control Unt12 qPCR primer set.)
Chromatin Immunoprecipitation (ChIP)
(AbFlex AM-Tag antibody (rAb) tested by ChIP. ’s pAM_1C_JunD Vector was transiently transfected or mock transfected into HCT116 cells. Chromatin was harvested according to the instructions in the Tag-ChIP-IT Kit . 10 ug of the AM-Tag antibody was used to immunoprecipitate the cross-linked AM-Tag-JunD fusion protein. qPCR data shows enrichment of AM-Tag-JunD with the FOXRED qPCR primer set and little to no enrichment in the mock transfections or when using the negative control Unt12 qPCR primer set.)
Western Blot (WB)
(AbFlex AM-Tag antibody (rAb) tested by Western Blot. ’s pAM_1C_JunD Vector was transfected or mock transfected into HCT116 cells. 48 hours post-transfection nuclear lysates were prepared. 15 ug lysate was loaded per well. Western blot was performed using 1 ug/mL of AM-Tag antibody.)
Western Blot (WB)
(AbFlex AM-Tag antibody (rAb) tested by Western Blot. ’s pAM_1C_JunD Vector was transfected or mock transfected into HCT116 cells. 48 hours post-transfection nuclear lysates were prepared. 15 ug lysate was loaded per well. Western blot was performed using 1 ug/mL of AM-Tag antibody.)
Related Product Information for anti-AM antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex AM-Tag antibody (rAb) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ELISA and Western blot; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex AM-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The AM-Tag antibody is specifically designed for use with Our unique AM-Tag sequence in combination with the Tag-ChIP-IT Kit. Simply clone your protein of interest into Our pAM_1C Empty Vector, or add the AM-Tag sequence to the C-terminus of your protein expression vector. Following transfection, cell lysates are prepared and screened by Western blot for expression of the AM-Tag fusion protein using the AM-Tag antibody. Optimization may be required to identify the optimal conditions for transfection and protein expression. Once transfection conditions have been optimized, the Tag-ChIP-IT Kit can be used to isolate chromatin and perform ChIP. The AM-Tag has minimal cross reactivity with mammalian samples to ensure low background signal. The tag is also unstructured, which allows the tag to protrude from the protein of interest for maximum exposure during immunoprecipitation. This increases the enrichment efficiency of low abundance transcription factors for more reliable and consistent ChIP results. Use of the AM-Tag for chromatin immunoprecipitation avoids the need for protein-specific ChIP-validated antibodies. It also enables the analysis of sequence variants, mutations and truncations on gene regulation.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex AM-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The AM-Tag antibody is specifically designed for use with Our unique AM-Tag sequence in combination with the Tag-ChIP-IT Kit. Simply clone your protein of interest into Our pAM_1C Empty Vector, or add the AM-Tag sequence to the C-terminus of your protein expression vector. Following transfection, cell lysates are prepared and screened by Western blot for expression of the AM-Tag fusion protein using the AM-Tag antibody. Optimization may be required to identify the optimal conditions for transfection and protein expression. Once transfection conditions have been optimized, the Tag-ChIP-IT Kit can be used to isolate chromatin and perform ChIP. The AM-Tag has minimal cross reactivity with mammalian samples to ensure low background signal. The tag is also unstructured, which allows the tag to protrude from the protein of interest for maximum exposure during immunoprecipitation. This increases the enrichment efficiency of low abundance transcription factors for more reliable and consistent ChIP results. Use of the AM-Tag for chromatin immunoprecipitation avoids the need for protein-specific ChIP-validated antibodies. It also enables the analysis of sequence variants, mutations and truncations on gene regulation.
Product Categories/Family for anti-AM antibody
NCBI and Uniprot Product Information
Molecular Weight
15kDa
Protein Family
Similar Products
Product Notes
The AM (Catalog #AAA389441) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex AM-Tag antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AM can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), ELISA (EIA), Western Blot (WB). Applications Validated: ChIP: 10ug per ChIP WB: 0.5-2ug/ml dilution Bead-based ELISA: 7-560 ng/ml. Researchers should empirically determine the suitability of the AM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AM, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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