Rabbit anti-Human CTCF Antibody | anti-CTCF antibody

AbFlex CTCF antibody (rAb)

Cat. Num. AAA389498

• Gene Names: CTCF; MRD21
• Reactivity: Human
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
• Purity: Protein A Chromatography
• Synonyms: CTCF; AbFlex CTCF antibody (rAb); CCCTC-Binding Factor; MRD21; anti-CTCF antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Protein A Chromatography
• Form/Format: Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-CTCF antibody: Chromatin Immunoprecipitation-Seq (ChIP-Seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Western Blot (WB)
• Application Notes: Applications Validated:; ChIP-Seq: 4ug per ChIP; WB: 0.1-1ug/ml; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts.
• Antibody Type: Recombinant Antibody
• Immunogen: This antibody was raised against a peptide within human CTCF.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Chromatin Immunoprecipitation (ChIP)

(AbFlex CTCF recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of MCF7 chromatin and 4 ug of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to identify CTCF binding sites on chromosome 9.)

Western Blot (WB)

(AbFlex CTCF antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex CTCF antibody at 0.2 ug/ml.)

Testing Data

(AbFlex CTCF recombinant antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 250,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NextSeq 500/550. Data was collected from 21 million reads, and CTCF data is shown for Chromosome 1.)

Related Product Information for anti-CTCF antibody

Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex CTCF antibody (rAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq, CUT&Tag and Western blot; it has been shown to react with Human samples.

Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CTCF antibody was expressed in CHO cells and contains rabbit immunoglobulin heavy and light chains. CTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. It is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping.

Product Categories/Family for anti-CTCF antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5729790
• NCBI GeneID: 10664
• NCBI Accession #: NP_006556
• NCBI GenBank Nucleotide #: NM_006565
• UniProt Accession #: P49711
• Molecular Weight: 83kDa
• NCBI Official Full Name: transcriptional repressor CTCF isoform 1
• NCBI Official Synonym Full Names: CCCTC-binding factor
• NCBI Official Symbol: CTCF
• NCBI Official Synonym Symbols: MRD21
• NCBI Protein Information: transcriptional repressor CTCF
• UniProt Protein Name: Transcriptional repressor CTCF
• Protein Family: Transcriptional repressor
• UniProt Gene Name: CTCF
• UniProt Entry Name: CTCF_HUMAN

NCBI Description

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

CTCF: a widely expressed, sequence-specific DNA-binding transcriptional regulator, insulator, and organizer of higher-order chromatin structure. Acts as a tumor suppressor. Contains 11 C2H2-type zinc fingers. Involved in promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mediates pairing between X chromosomes and interactions between distant regulatory elements. Binds to promoters of c-myc, PLK, PIM1 and APP. A critical regulator of cell-cycle arrest and death after B cell receptor signaling in immature B cells. CTCF, together with YY1 and Tsix, form a regulated epigenetic switch for X-inactivation.

Protein type: Tumor suppressor; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 16q21-q22.3

Cellular Component: nucleoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; chromatin insulator sequence binding; transcription factor activity; transcription corepressor activity

Biological Process: genetic imprinting; transcription, DNA-dependent; maintenance of DNA methylation; positive regulation of transcription, DNA-dependent; regulation of histone methylation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of gene expression, epigenetic; nucleosome positioning; chromosome segregation; regulation of molecular function, epigenetic; DNA methylation; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 21