Immunoprecipitation (IP)
(AbFlex Ago1/2/3 antibody (rAb) tested antibody tested by immunoprecipitation. miRNA IP Kit was used with 293 cells (10 million cells per IP). Following IP using AbFlex Ago1/2/3 antibody (5 ug per IP) or a negative control IgG, qRT-PCR was performed on samples using primers for IGF2. Fold enrichment over the negative control IgG shows successful specific IP of IGF2 miRNA associated with the RISC complex.)
Mouse anti-Human Ago 1/2/3 Antibody | anti-Ago1/2/3 antibody
AbFlex Ago 1/2/3 antibody (rAb)
Reactivity
Human
Applications
Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Ago 1/2/3; AbFlex Ago 1/2/3 antibody (rAb); Argonaute; RISC catalog component; anti-Ago1/2/3 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Ago1/2/3 antibody
Immunoprecipitation (IP)
Application Notes
Applications Validated:
IP: 5ug per sample AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
IP: 5ug per sample AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This Ago1/2/3 antibody was raised against a recombinant protein corresponding to amino acids 47-859 of human Ago2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Immunoprecipitation (IP)
(AbFlex Ago1/2/3 antibody (rAb) tested antibody tested by immunoprecipitation. miRNA IP Kit was used with 293 cells (10 million cells per IP). Following IP using AbFlex Ago1/2/3 antibody (5 ug per IP) or a negative control IgG, qRT-PCR was performed on samples using primers for IGF2. Fold enrichment over the negative control IgG shows successful specific IP of IGF2 miRNA associated with the RISC complex.)
Immunoprecipitation (IP)
(AbFlex Ago1/2/3 antibody (rAb) tested antibody tested by immunoprecipitation. miRNA IP Kit was used with 293 cells (10 million cells per IP). Following IP using AbFlex Ago1/2/3 antibody (5 ug per IP) or a negative control IgG, qRT-PCR was performed on samples using primers for IGF2. Fold enrichment over the negative control IgG shows successful specific IP of IGF2 miRNA associated with the RISC complex.)
Related Product Information for anti-Ago1/2/3 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Ago 1/2/3 antibody (rAb) was raised in a Mouse host. It has been validated for use in Immunoprecipitation; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Ago 1/2/3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The Argonaute family of proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Ago3 lacks RNase activity and does not appear to cleave target mRNA molecules. Argonaute family proteins contain a PAZ and a PIWI domain.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Ago 1/2/3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The Argonaute family of proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Ago3 lacks RNase activity and does not appear to cleave target mRNA molecules. Argonaute family proteins contain a PAZ and a PIWI domain.
Product Categories/Family for anti-Ago1/2/3 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Ago1/2/3 (Catalog #AAA389472) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Ago 1/2/3 antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ago 1/2/3 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Applications Validated: IP: 5ug per sample AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the Ago1/2/3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ago 1/2/3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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