Mouse GSK3beta (Glycogen synthase kinase-3 beta) ELISA Kit | GSK3beta elisa kit

Mouse GSK3beta (Glycogen synthase kinase-3 beta) ELISA Kit

Cat. Num. AAA7606180

• Gene Names: Gsk3b; GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
• Reactivity: Mouse
• Synonyms: GSK3beta (Glycogen synthase kinase-3 beta); Mouse GSK3beta (Glycogen synthase kinase-3 beta) ELISA Kit; GSK3beta; Glycogen Synthase Kinase 3 Beta; GSK3B; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; GSK3beta elisa kit

• Reactivity: Mouse
• Sequence Length: 420

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.15-30ng/ml
• Sensitivity: 0.133ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSK3beta elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GSK3?antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GSK3? antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GSK3? amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GSK3? can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 11133187
• NCBI GeneID: 56637
• UniProt Accession #: Q9WV60
• Molecular Weight: 46,710 Da
• NCBI Official Full Name: Glycogen synthase kinase-3 beta
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: Gsk3b
• NCBI Official Synonym Symbols: GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: Gsk3b
• UniProt Synonym Gene Names: GSK-3 beta

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: CMGC group; EC 2.7.11.1; EC 2.7.11.26; GSK family; GSK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16|16 B3

Cellular Component: axon; beta-catenin destruction complex; cell soma; centrosome; cytoplasm; cytosol; dendrite; dendritic shaft; dendritic spine; growth cone; lipid raft; membrane; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; protein complex; ribonucleoprotein complex

Molecular Function: ATP binding; beta-catenin binding; integrin binding; ionotropic glutamate receptor binding; kinase activity; NF-kappaB binding; p53 binding; protease binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau protein binding; tau-protein kinase activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: axonogenesis; cell migration; cell proliferation; circadian rhythm; cytoskeleton organization and biogenesis; epithelial to mesenchymal transition; ER overload response; establishment and/or maintenance of cell polarity; establishment of cell polarity; fat cell differentiation; genetic imprinting; glycogen metabolic process; hippocampus development; insulin receptor signaling pathway; myoblast fusion; myotube differentiation; negative regulation of apoptosis; negative regulation of dendrite morphogenesis; negative regulation of MAP kinase activity; negative regulation of neuron maturation; negative regulation of NFAT protein import into nucleus; negative regulation of nitric-oxide synthase activity; negative regulation of protein binding; negative regulation of protein complex assembly; negative regulation of TOR signaling pathway; organ morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of axon extension; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein export from nucleus; re-entry into mitotic cell cycle; regulation of axon extension; regulation of axonogenesis; regulation of dendrite morphogenesis; regulation of microtubule-based process; regulation of neuronal synaptic plasticity; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin