Human GSK3beta (Glycogen Synthase Kinase 3 Beta) CLIA Kit | GSK3b clia kit

Human GSK3beta (Glycogen Synthase Kinase 3 Beta) CLIA Kit

Cat. Num. AAA2531120

• Reactivity: Human
• Synonyms: GSK3beta (Glycogen Synthase Kinase 3 Beta); Human GSK3beta (Glycogen Synthase Kinase 3 Beta) CLIA Kit; Human GSK3b (Glycogen Synthase Kinase 3 Beta) CLIA Kit; Human GSK3b; GSK3b clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant GSK3beta. No significant cross-reactivity or interference between GSK3beta and analogues was observed.
• Sequence Length: 28

• Assay Type: Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: Min: 75pg/mL; Max: 8000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSK3b clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of GSK3beta concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GSK3beta. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GSK3beta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GSK3beta, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GSK3beta. You can calculate the concentration of GSK3beta in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5730335
• NCBI GeneID: 2932
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: glycogen synthase kinase 3 beta, partial
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: EC 2.7.11.1; Kinase, protein; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: nucleoplasm; centrosome; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; cytosol; nucleus; beta-catenin destruction complex; ribonucleoprotein complex

Molecular Function: protein serine/threonine kinase activity; NF-kappaB binding; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; protein kinase binding; ATP binding

Biological Process: circadian rhythm; glycogen metabolic process; fat cell differentiation; axon guidance; genetic imprinting; re-entry into mitotic cell cycle; nerve growth factor receptor signaling pathway; positive regulation of protein binding; positive regulation of axon extension; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; negative regulation of protein binding; positive regulation of cell-matrix adhesion; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; negative regulation of neuron maturation; organ morphogenesis; peptidyl-serine phosphorylation; positive regulation of protein complex assembly; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; positive regulation of GTPase activity; negative regulation of apoptosis