IL12 active protein

IL12, 23-328/23-219aa, Human, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA205947

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: IL12; 23-328/23-219aa; Human; His tag; Baculovirus (Bioactivity Validated); Interleukin 12 (subunit beta/alpha); NKSF1; IL12 active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: * IL-12 is a heterodimer of IL12A and IL12B linked through a disulfide-bond between cysteines bolded in below sequences.; IL12A (p35); RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH; IL12B (p40); IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
• Sequence Length: 253

• Applicable Applications for IL12 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less than or equal to 0.03 ng/ml.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IL12 active protein

IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.

Product Categories/Family for IL12 active protein

Hormones & Cytokines

References

Barton J.L., et al. (2000) Eur. J. Immunol. 30:3299-3308; Bazan J.F., et al. (2001) J. Immunol. 167:1440-1446

NCBI and Uniprot Product Information

• NCBI GI #: 24430219
• NCBI GeneID: 3592
• NCBI Accession #: NP_000873; NP_002178
• NCBI GenBank Nucleotide #: NM_000882.3
• UniProt Accession #: P29459; Q96QZ1
• Molecular Weight: 34.6kDa (p40) & 23.3kDa (p35) (306aa & 203aa) The heterodimeric IL12 has a predicted molecular weight of 57.9kDa (509aa). Molecular weight on SDS-PAGE will appear higher.
• NCBI Official Full Name: interleukin-12 subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.