Human Glycogen Synthase Kinase 3 beta ELISA Kit | GSK 3beta elisa kit

Human Glycogen Synthase Kinase 3 beta ELISA Kit

Cat. Num. AAA7253535

• Reactivity: Human
• Synonyms: Glycogen Synthase Kinase 3 beta; Human Glycogen Synthase Kinase 3 beta ELISA Kit; Human Glycogen Synthase Kinase 3 b ELISA Kit; GSK 3beta elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for GSK 3beta elisa kit

Intended Uses: This GSK3beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GSK3beta. This ELISA kit for research use only!

Principle of the Assay: GSK3beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSK3beta antibody and an GSK3beta -HRP conjugate. The assay sample and buffer are incubated together with GSK3beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSK3beta concentration since GSK3beta from samples and GSK3beta -HRP conjugate compete for the anti-GSK3beta antibody binding site. Since the number of sites is limited, as more sites are occupied by GSK3beta from the sample, fewer sites are left to bind GSK3beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3beta concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20455502
• NCBI GeneID: 2932
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: Glycogen synthase kinase-3 beta
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: EC 2.7.11.1; Protein kinase, CMGC; Kinase, protein; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: centrosome; dendritic spine; beta-catenin destruction complex; ribonucleoprotein complex; cytosol; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: integrin binding; protein serine/threonine kinase activity; ionotropic glutamate receptor binding; NF-kappaB binding; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; protein kinase binding; tau protein binding; ATP binding

Biological Process: fat cell differentiation; glycogen metabolic process; circadian rhythm; axon guidance; negative regulation of MAP kinase activity; genetic imprinting; re-entry into mitotic cell cycle; positive regulation of protein binding; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; response to lithium ion; positive regulation of axon extension; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; negative regulation of protein binding; positive regulation of cell-matrix adhesion; myoblast fusion; ER overload response; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; negative regulation of neuron maturation; organ morphogenesis; peptidyl-serine phosphorylation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; regulation of neuronal synaptic plasticity; negative regulation of apoptosis